P2X7 mediates ATP-driven invasiveness in prostate cancer cells

The ATP-gated P2X7 has been shown to play an important role in invasiveness and metastasis of some tumors. However, the possible links and underlying mechanisms between P2X7 and prostate cancer have not been elucidated. Here, we demonstrated that P2X7 was highly expressed in some prostate cancer cel...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2014-12, Vol.9 (12), p.e114371
Main Authors: Qiu, Ying, Li, Wei-Hua, Zhang, Hong-Quan, Liu, Yan, Tian, Xin-Xia, Fang, Wei-Gang
Format: Article
Language:English
Subjects:
1-Phosphatidylinositol 3-kinase
Adenosine Triphosphate - metabolism
AKT protein
Angiogenesis
Animals
ATP
Biology and Life Sciences
Cancer
Cancer cells
Cell growth
Cell Line, Tumor
Cell Movement - genetics
Cytokines
Dendritic cells
Disease Models, Animal
E-cadherin
Education
Epithelial-Mesenchymal Transition - genetics
Gene Expression
Gene Knockdown Techniques
Genes
Humans
Immunoglobulins
Interleukin 8
Invasiveness
Laboratories
Male
MAP Kinase Signaling System
Medicine and Health Sciences
Metastases
Metastasis
Mice
Neoplasm Metastasis
Pathology
Phosphatidylinositol 3-Kinases - metabolism
Phosphorylation
Prostate cancer
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
Proto-Oncogene Proteins c-akt - metabolism
Receptors, Purinergic P2X7 - genetics
Receptors, Purinergic P2X7 - metabolism
Science
siRNA
Therapeutic applications
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The ATP-gated P2X7 has been shown to play an important role in invasiveness and metastasis of some tumors. However, the possible links and underlying mechanisms between P2X7 and prostate cancer have not been elucidated. Here, we demonstrated that P2X7 was highly expressed in some prostate cancer cells. Down-regulation of P2X7 by siRNA significantly attenuated ATP- or BzATP-driven migration and invasion of prostate cancer cells in vitro, and inhibited tumor invasiveness and metastases in nude mice. In addition, silencing of P2X7 remarkably attenuated ATP- or BzATP- driven expression changes of EMT/invasion-related genes Snail, E-cadherin, Claudin-1, IL-8 and MMP-3, and weakened the phosphorylation of PI3K/AKT and ERK1/2 in vitro. Similar effects were observed in nude mice. These data indicate that P2X7 stimulates cell invasion and metastasis in prostate cancer cells via some EMT/invasion-related genes, as well as PI3K/AKT and ERK1/2 signaling pathways. P2X7 could be a promising therapeutic target for prostate cancer.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0114371