MiR-203 suppresses ZNF217 upregulation in colorectal cancer and its oncogenicity

Zinc finger protein 217 (ZNF217) is essential for cell proliferation and has been implicated in tumorigenesis. However, its expression and exact roles in colorectal cancer (CRC) remain unclear. In this study, we demonstrated that ZNF217 expression was aberrantly upregulated in CRC tissues and associ...

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Bibliographic Details
Published in:PloS one 2015-01, Vol.10 (1), p.e0116170
Main Authors: Li, Zewu, Du, Lutao, Dong, Zhaogang, Yang, Yongmei, Zhang, Xin, Wang, Lili, Li, Juan, Zheng, Guixi, Qu, Ailin, Wang, Chuanxin
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Aberration
Analysis
Bioinformatics
Cancer
Carcinogenesis - genetics
Carcinogenesis - metabolism
Cell cycle
Cell migration
Cell Movement
Cell Proliferation
Chromosomes
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - genetics
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - mortality
Combined treatment
Cytoplasm
Female
Gene Expression Regulation, Neoplastic
Genes
HT29 Cells
Humans
Laboratories
Luciferase
Male
Metastasis
MicroRNA
MicroRNAs
MicroRNAs - physiology
Middle Aged
miRNA
Neoplasm Invasiveness
Prostate
Proteins
Ribonucleic acid
RNA
siRNA
Tissues
Trans-Activators - genetics
Trans-Activators - metabolism
Tumorigenesis
Up-Regulation
Zinc
Zinc finger proteins
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Summary:Zinc finger protein 217 (ZNF217) is essential for cell proliferation and has been implicated in tumorigenesis. However, its expression and exact roles in colorectal cancer (CRC) remain unclear. In this study, we demonstrated that ZNF217 expression was aberrantly upregulated in CRC tissues and associated with poor overall survival of CRC patients. In addition, we found that ZNF217 was a putative target of microRNA (miR)-203 using bioinformatics analysis and confirmed that using luciferase reporter assay. Moreover, in vitro knockdown of ZNF217 or enforced expression of miR-203 attenuated CRC cell proliferation, invasion and migration. Furthermore, combined treatment of ZNF217 siRNA and miR-203 exhibited synergistic inhibitory effects. Taken together, our results provide new evidences that ZNF217 has an oncogenic role in CRC and is regulated by miR-203, and open up the possibility of ZNF217- and miR-203-targeted therapy for CRC.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0116170