Photo-attachment of biomolecules for miniaturization on wicking Si-nanowire platform

We demonstrated the surface functionalization of a highly three-dimensional, superhydrophilic wicking substrate using light to immobilize functional biomolecules for sensor or microarray applications. We showed here that the three-dimensional substrate was compatible with photo-attachment and the pe...

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Bibliographic Details
Published in:PloS one 2015-02, Vol.10 (2), p.e0116539-e0116539
Main Authors: Cheng, He, Zheng, Han, Wu, Jia Xin, Xu, Wei, Zhou, Lihan, Leong, Kam Chew, Fitzgerald, Eugene, Rajagopalan, Raj, Too, Heng Phon, Choi, Wee Kiong
Format: Article
Language:English
Subjects:
Attachment
Biochemistry
Biomolecules
Biosensors
Biotechnology
Carbocyanines - chemistry
Chemicals
Computer engineering
Deoxyribonucleic acid
DNA
DNA Probes - metabolism
Fabrication
Hybridization
Hydrophobic surfaces
Immobilized Nucleic Acids - chemistry
Immobilized Nucleic Acids - metabolism
Microarray Analysis
MicroRNAs - chemistry
MicroRNAs - metabolism
Miniaturization
Nanotechnology
Nanowires
Nanowires - chemistry
Nucleic Acid Hybridization
Nucleic Acids - chemistry
Nucleic Acids - metabolism
Silicon - chemistry
Substrates
Surface Properties
Target detection
Ultraviolet Rays
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Summary:We demonstrated the surface functionalization of a highly three-dimensional, superhydrophilic wicking substrate using light to immobilize functional biomolecules for sensor or microarray applications. We showed here that the three-dimensional substrate was compatible with photo-attachment and the performance of functionalization was greatly improved due to both increased surface capacity and reduced substrate reflectivity. In addition, photo-attachment circumvents the problems induced by wicking effect that was typically encountered on superhydrophilic three-dimensional substrates, thus reducing the difficulty of producing miniaturized sites on such substrate. We have investigated various aspects of photo-attachment process on the nanowire substrate, including the role of different buffers, the effect of wavelength as well as how changing probe structure may affect the functionalization process. We demonstrated that substrate fabrication and functionalization can be achieved with processes compatible with microelectronics processes, hence reducing the cost of array fabrication. Such functionalization method coupled with the high capacity surface makes the substrate an ideal candidate for sensor or microarray for sensitive detection of target analytes.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0116539