Monoclonal antibodies directed to fucoidan preparations from brown algae

Cell walls of the brown algae contain a diverse range of polysaccharides with useful bioactivities. The precise structures of the sulfated fucan/fucoidan group of polysaccharides and their roles in generating cell wall architectures and cell properties are not known in detail. Four rat monoclonal an...

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Published in:PloS one 2015-02, Vol.10 (2), p.e0118366-e0118366
Main Authors: Torode, Thomas A, Marcus, Susan E, Jam, Murielle, Tonon, Thierry, Blackburn, Richard S, Hervé, Cécile, Knox, J Paul
Format: Article
Language:English
Subjects:
Acids
Algae
Animals
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - metabolism
Antigenic determinants
Biology
Cell adhesion & migration
Cell Wall - metabolism
Cell walls
Cellulose
Chromatography, Ion Exchange
Distribution patterns
Epitope Mapping
Epitopes
Fluorescence
Fucoidan
Fucus
Heterogeneity
Immunoglobulins
Life Sciences
Male
Monoclonal antibodies
Phaeophyceae
Phaeophyceae - chemistry
Phaeophyceae - classification
Plant sciences
Polymers
Polysaccharides
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Rats
Rats, Wistar
Saccharides
Salinity
Sulfates
Tissue analysis
Tissues
Vegetal Biology
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cited_by cdi_FETCH-LOGICAL-c726t-169cb3d9fa6f3bf8be3d2e7948293a59f7ce47f638fb177337798cbb793476873
cites cdi_FETCH-LOGICAL-c726t-169cb3d9fa6f3bf8be3d2e7948293a59f7ce47f638fb177337798cbb793476873
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container_issue 2
container_start_page e0118366
container_title PloS one
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creator Torode, Thomas A
Marcus, Susan E
Jam, Murielle
Tonon, Thierry
Blackburn, Richard S
Hervé, Cécile
Knox, J Paul
description Cell walls of the brown algae contain a diverse range of polysaccharides with useful bioactivities. The precise structures of the sulfated fucan/fucoidan group of polysaccharides and their roles in generating cell wall architectures and cell properties are not known in detail. Four rat monoclonal antibodies, BAM1 to BAM4, directed to sulfated fucan preparations, have been generated and used to dissect the heterogeneity of brown algal cell wall polysaccharides. BAM1 and BAM4, respectively, bind to a non-sulfated epitope and a sulfated epitope present in the sulfated fucan preparations. BAM2 and BAM3 identified additional distinct epitopes present in the fucoidan preparations. All four epitopes, not yet fully characterised, occur widely within the major brown algal taxonomic groups and show divergent distribution patterns in tissues. The analysis of cell wall extractions and fluorescence imaging reveal differences in the occurrence of the BAM1 to BAM4 epitopes in various tissues of Fucus vesiculosus. In Ectocarpus subulatus, a species closely related to the brown algal model Ectocarpus siliculosus, the BAM4 sulfated epitope was modulated in relation to salinity levels. This new set of monoclonal antibodies will be useful for the dissection of the highly complex and yet poorly resolved sulfated polysaccharides in the brown algae in relation to their ecological and economic significance.
doi_str_mv 10.1371/journal.pone.0118366
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In Ectocarpus subulatus, a species closely related to the brown algal model Ectocarpus siliculosus, the BAM4 sulfated epitope was modulated in relation to salinity levels. This new set of monoclonal antibodies will be useful for the dissection of the highly complex and yet poorly resolved sulfated polysaccharides in the brown algae in relation to their ecological and economic significance.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25692870</pmid><doi>10.1371/journal.pone.0118366</doi><orcidid>https://orcid.org/0000-0001-6649-8137</orcidid><oa>free_for_read</oa></addata></record>
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subjects Acids
Algae
Animals
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - metabolism
Antigenic determinants
Biology
Cell adhesion & migration
Cell Wall - metabolism
Cell walls
Cellulose
Chromatography, Ion Exchange
Distribution patterns
Epitope Mapping
Epitopes
Fluorescence
Fucoidan
Fucus
Heterogeneity
Immunoglobulins
Life Sciences
Male
Monoclonal antibodies
Phaeophyceae
Phaeophyceae - chemistry
Phaeophyceae - classification
Plant sciences
Polymers
Polysaccharides
Polysaccharides - chemistry
Polysaccharides - isolation & purification
Rats
Rats, Wistar
Saccharides
Salinity
Sulfates
Tissue analysis
Tissues
Vegetal Biology
title Monoclonal antibodies directed to fucoidan preparations from brown algae
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