Malassezia intra-specific diversity and potentially new species in the skin microbiota from Brazilian healthy subjects and seborrheic dermatitis patients

• Published in: PloS one 2015-02, Vol.10 (2), p.e0117921-e0117921
• Main Authors: Soares, Renan Cardoso, Zani, Marcelo Bergamin, Arruda, Ana Carolina Belini Bazán, Arruda, Lucia Helena Fávaro de, Paulino, Luciana Campos
• Format: Article
• Language: English
• Subjects: Analysis; Biodiversity; Brazil; Deoxyribonucleic acid; Dermatitis; Dermatitis, Seborrheic - microbiology; Discovery and exploration; Diseases; DNA; Forehead; Fungi; Genetic diversity; Genetic Variation; Healthy Volunteers; Hospitals; Humans; Lesions; Malassezia; Malassezia - genetics; Malassezia - isolation & purification; Malassezia globosa; Malassezia pachydermatis; Microbiota; Microbiota (Symbiotic organisms); Molecular chains; Multiculturalism & pluralism; New species; Patients; Phylogeny; Physical characteristics; Scalp; Seborrheic dermatitis; Skin; Skin - microbiology; Skin diseases; Species diversity; Taxonomy; Yeasts
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0117921

Malassezia yeasts are part of the resident cutaneous microbiota, and are also associated with skin diseases such as seborrheic dermatitis (SD). The role these fungi play in skin diseases and why they are pathogenic for only some individuals remain unclear. This study aimed to characterize Malassezia microbiota from different body sites in healthy and SD subjects from Brazil. Scalp and forehead samples from healthy, mild SD and severe SD subjects were collected. Non-scalp lesions from severe SD patients were also sampled. 5.8S rDNA/ITS2 amplicons from Malassezia sp. were analyzed by RFLP and sequencing. Results indicate that Malassezia microbiota did not group according to health condition or body area. Phylogenetic analysis revealed that three groups of sequences did not cluster together with any formally described species, suggesting that they might belong to potential new species. One of them was found in high proportions in scalp samples. A large variety of Malassezia subtypes were detected, indicating intra-specific diversity. Higher M. globosa proportions were found in non-scalp lesions from severe SD subjects compared with other areas, suggesting closer association of this species with SD lesions from areas other than scalp. Our results show the first panorama of Malassezia microbiota in Brazilian subjects using molecular techniques and provide new perspectives for further studies to elucidate the association between Malassezia microbiota and skin diseases.