Pathogenic chytrid fungus Batrachochytrium dendrobatidis, but not B. salamandrivorans, detected on eastern hellbenders

• Published in: PloS one 2015-02, Vol.10 (2), p.e0116405-e0116405
• Main Authors: Bales, Emma K, Hyman, Oliver J, Loudon, Andrew H, Harris, Reid N, Lipps, Gregory, Chapman, Eric, Roblee, Kenneth, Kleopfer, John D, Terrell, Kimberly A
• Format: Article
• Language: English
• Subjects: Amphibia; Amphibians; Amphibians - microbiology; Andrias; Animals; Aquatic environment; Batrachochytrium dendrobatidis; Biology; Casualties; Chytridiomycosis; Chytridiomycota - pathogenicity; Confidence intervals; Conservation; Cryptobranchidae; Disease; Extinct species; Fitness; Fungi; Genomes; Host range; Infections; Pathogens; Population studies; Population viability; Populations; Reptiles & amphibians; Skin; Skin diseases; Species; Surveys; United States; Urodela - microbiology; Zoospores
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0116405

Recent worldwide declines and extinctions of amphibian populations have been attributed to chytridiomycosis, a disease caused by the pathogenic fungus Batrachochytrium dendrobatidis (Bd). Until recently, Bd was thought to be the only Batrachochytrium species that infects amphibians; however a newly described species, Batrachochytrium salamandrivorans (Bs), is linked to die-offs in European fire salamanders (Salamandra salamandra). Little is known about the distribution, host range, or origin of Bs. In this study, we surveyed populations of an aquatic salamander that is declining in the United States, the eastern hellbender (Cryptobranchus alleganiensis alleganiensis), for the presence of Bs and Bd. Skin swabs were collected from a total of 91 individuals in New York, Pennsylvania, Ohio, and Virginia, and tested for both pathogens using duplex qPCR. Bs was not detected in any samples, suggesting it was not present in these hellbender populations (0% prevalence, 95% confidence intervals of 0.0-0.04). Bd was found on 22 hellbenders (24% prevalence, 95% confidence intervals of 0.16 ≤ 0.24 ≤ 0.34), representing all four states. All positive samples had low loads of Bd zoospores (12.7 ± 4.9 S.E.M. genome equivalents) compared to other Bd susceptible species. More research is needed to determine the impact of Batrachochytrium infection on hellbender fitness and population viability. In particular, understanding how hellbenders limit Bd infection intensity in an aquatic environment may yield important insights for amphibian conservation. This study is among the first to evaluate the distribution of Bs in the United States, and is consistent with another, which failed to detect Bs in the U.S. Knowledge about the distribution, host-range, and origin of Bs may help control the spread of this pathogen, especially to regions of high salamander diversity, such as the eastern United States.