Genome-wide identification of small RNAs in Bifidobacterium animalis subsp. lactis KLDS 2.0603 and their regulation role in the adaption to gastrointestinal environment

Bifidobacteria are one of the predominant bacterial species in the human gastrointestinal tract (GIT) and play a vital role in the host's health by acting as probiotics. However, how they regulate themselves to adapt to GIT of their host remains unknown. Eighteen bifidobacterial strains were us...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-02, Vol.10 (2), p.e0117373
Main Authors: Zhu, De-Quan, Liu, Fei, Sun, Yu, Yang, Li-Mei, Xin, Li, Meng, Xiang-Chen
Format: Article
Language:English
Subjects:
Adaptation, Physiological - genetics
Adhesion
Bacillus subtilis
Bacteria
Bifidobacterium
Bifidobacterium - genetics
Bifidobacterium animalis
Bioinformatics
Cell adhesion & migration
Cellulose
E coli
Education
Environmental changes
Escherichia coli
Food safety
Gastrointestinal tract
Gastrointestinal Tract - microbiology
Gene expression
Genes
Genome, Bacterial
Genomes
Gram-positive bacteria
Humans
Laboratories
Molecular biology
Nucleotide sequence
Nutrition
Post-transcription
Probiotics
RNA, Bacterial
Science
Strain
Streptococcus infections
Survival
Transcriptome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bifidobacteria are one of the predominant bacterial species in the human gastrointestinal tract (GIT) and play a vital role in the host's health by acting as probiotics. However, how they regulate themselves to adapt to GIT of their host remains unknown. Eighteen bifidobacterial strains were used to analyze their adaptive capacities towards simulated GIT environment. The strain with highest survival rate and adhesion ability was selected for comparative genome as well as transcriptomic analysis. The Bifidobacterium animalis subsp. lactis KLDS 2.0603 strain was demonstrated to have the highest survival rate and adhesion ability in simulated GIT treatments. The comparative genome analysis revealed that the KLDS 2.0603 has most similar whole genome sequence compared with BB-12 strain. Eleven intergenic sRNAs were identified after genomes prediction and transcriptomic analysis of KLDS 2.0603. Transcriptomic analysis also showed that genes (mainly sRNAs targeted genes) and sRNAs were differentially expressed in different stress conditions, suggesting that sRNAs might play a crucial role in regulating genes involved in the stress resistance of this strain towards environmental changes. This study first provided deep and comprehensive insights into the regulation of KLDS 2.0603 strain at transcription and post-transcription level towards environmental.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0117373