Synergistic antimicrobial activity of Camellia sinensis and Juglans regia against multidrug-resistant bacteria

Synergistic combinations of antimicrobial agents with different mechanisms of action have been introduced as more successful strategies to combat infections involving multidrug resistant (MDR) bacteria. In this study, we investigated synergistic antimicrobial activity of Camellia sinensis and Juglan...

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Published in:PloS one 2015-02, Vol.10 (2), p.e0118431-e0118431
Main Authors: Farooqui, Amber, Khan, Adnan, Borghetto, Ilaria, Kazmi, Shahana U, Rubino, Salvatore, Paglietti, Bianca
Format: Article
Language:English
Subjects:
Agar
Anti-Bacterial Agents - pharmacology
Antibacterial activity
Antibacterial agents
Antimicrobial activity
Antimicrobial agents
Bacteria
Camellia sinensis
Camellia sinensis - chemistry
Catechin
Cell morphology
Cell walls
Cytology
Dilution
Drug resistance
Drug Synergism
Electron microscopy
Escherichia coli
Gram-positive bacteria
Health aspects
Juglans - chemistry
Juglans regia
Kinetics
Laboratories
Methicillin
Methicillin-Resistant Staphylococcus aureus - drug effects
Microbial drug resistance
Multidrug resistance
Multidrug resistant organisms
Nalidixic acid
Nalidixic Acid - pharmacology
Oxacillin
Oxacillin - pharmacology
Pathogens
Plant extracts
Plant Extracts - pharmacology
Salmonella
Salmonella enterica
Shigella
Staphylococcus aureus
Staphylococcus infections
Strains (organisms)
Tea
Titration
Transmission electron microscopy
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Khan, Adnan
Borghetto, Ilaria
Kazmi, Shahana U
Rubino, Salvatore
Paglietti, Bianca
description Synergistic combinations of antimicrobial agents with different mechanisms of action have been introduced as more successful strategies to combat infections involving multidrug resistant (MDR) bacteria. In this study, we investigated synergistic antimicrobial activity of Camellia sinensis and Juglans regia which are commonly used plants with different antimicrobial agents. Antimicrobial susceptibility of 350 Gram-positive and Gram-negative strains belonging to 10 different bacterial species, was tested against Camellia sinensis and Juglans regia extracts. Minimum inhibitory concentrations (MICs) were determined by agar dilution and microbroth dilution assays. Plant extracts were tested for synergistic antimicrobial activity with different antimicrobial agents by checkerboard titration, Etest/agar incorporation assays, and time kill kinetics. Extract treated and untreated bacteria were subjected to transmission electron microscopy to see the effect on bacterial cell morphology. Camellia sinensis extract showed higher antibacterial activity against MDR S. Typhi, alone and in combination with nalidixic acid, than to susceptible isolates." We further explore anti-staphylococcal activity of Juglans regia that lead to the changes in bacterial cell morphology indicating the cell wall of Gram-positive bacteria as possible target of action. The synergistic combination of Juglans regia and oxacillin reverted oxacillin resistance of methicillin resistant Staphylococcus aureus (MRSA) strains in vitro. This study provides novel information about antimicrobial and synergistic activity of Camellia sinensis and Juglans regia against MDR pathogens.
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We further explore anti-staphylococcal activity of Juglans regia that lead to the changes in bacterial cell morphology indicating the cell wall of Gram-positive bacteria as possible target of action. The synergistic combination of Juglans regia and oxacillin reverted oxacillin resistance of methicillin resistant Staphylococcus aureus (MRSA) strains in vitro. This study provides novel information about antimicrobial and synergistic activity of Camellia sinensis and Juglans regia against MDR pathogens.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25719410</pmid><doi>10.1371/journal.pone.0118431</doi><oa>free_for_read</oa></addata></record>
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1932-6203
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subjects Agar
Anti-Bacterial Agents - pharmacology
Antibacterial activity
Antibacterial agents
Antimicrobial activity
Antimicrobial agents
Bacteria
Camellia sinensis
Camellia sinensis - chemistry
Catechin
Cell morphology
Cell walls
Cytology
Dilution
Drug resistance
Drug Synergism
Electron microscopy
Escherichia coli
Gram-positive bacteria
Health aspects
Juglans - chemistry
Juglans regia
Kinetics
Laboratories
Methicillin
Methicillin-Resistant Staphylococcus aureus - drug effects
Microbial drug resistance
Multidrug resistance
Multidrug resistant organisms
Nalidixic acid
Nalidixic Acid - pharmacology
Oxacillin
Oxacillin - pharmacology
Pathogens
Plant extracts
Plant Extracts - pharmacology
Salmonella
Salmonella enterica
Shigella
Staphylococcus aureus
Staphylococcus infections
Strains (organisms)
Tea
Titration
Transmission electron microscopy
title Synergistic antimicrobial activity of Camellia sinensis and Juglans regia against multidrug-resistant bacteria
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