Adaptive differentiation in seedling traits in a hybrid pine species complex, Pinus densata and its parental species, on the Tibetan Plateau

• Published in: PloS one 2015-03, Vol.10 (3), p.e0118501-e0118501
• Main Authors: Meng, Jingxiang, Mao, Jian-Feng, Zhao, Wei, Xing, Fangqian, Chen, Xinyu, Liu, Hao, Xing, Zhen, Wang, Xiao-Ru, Li, Yue
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological; Biological evolution; Biology; Breeding of animals; Chloroplasts; Climate change; Deoxyribonucleic acid; Differentiation; DNA; Ecology; Empirical analysis; Engineering; Environmental factors; Environmental impact; Evolution & development; Evolution, Molecular; Experiments; Fitness; Forestry; Genes, Plant; Genetic aspects; Genetic Speciation; Genetics; Growth rate; Habitats; High altitude; High-altitude environments; Hybrid seeds; Hybridization; Laboratories; Parents; Phenotype; Phylogeny; Phylogeography; Physiology; Pine; Pine trees; Pines; Pinus; Pinus - genetics; Pinus - growth & development; Pinus densata; Pinus tabuliformis; Pinus yunnanensis; Plant sciences; Reproductive fitness; Seedlings; Speciation; Species; Species Specificity; Tibet; Trees; Viability; Winter temperatures
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0118501

Evidence from molecular genetics demonstrates that Pinus densata is a natural homoploid hybrid originating from the parent species Pinus tabuliformis and Pinus yunnanensis, and ecological selection may have played a role in the speciation of P. densata. However, data on differentiation in adaptive traits in the species complex are scarce. In this study, we performed a common garden test on 16 seedling traits to examine the differences between P. densata and its parental species in a high altitude environment. We found that among the 16 analyzed traits, 15 were significantly different among the species. Pinus tabuliformis had much earlier bud set and a relatively higher bud set ratio but poorer seedling growth, and P. yunnanensis had opposite responses for the same traits. P. densata had the greatest fitness with higher viability and growth rates than the parents. The relatively high genetic contribution of seedling traits among populations suggested that within each species the evolutionary background is complex. The correlations between the seedling traits of a population within a species and the environmental factors indicated different impacts of the environment on species evolution. The winter temperature is among the most important climate factors that affected the fitness of the three pine species. Our investigation provides empirical evidence on adaptive differentiation among this pine species complex at seedling stages.