Unveiling TRPV1 spatio-temporal organization in live cell membranes

Transient Receptor Potential Vanilloid 1 (TRPV1) is a non-selective cation channel that integrates several stimuli into nociception and neurogenic inflammation. Here we investigated the subtle TRPV1 interplay with candidate membrane partners in live cells by a combination of spatio-temporal fluctuat...

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Bibliographic Details
Published in:PloS one 2015-03, Vol.10 (3), p.e0116900-e0116900
Main Authors: Storti, Barbara, Di Rienzo, Carmine, Cardarelli, Francesco, Bizzarri, Ranieri, Beltram, Fabio
Format: Article
Language:English
Subjects:
Animals
Capsaicin receptors
Caveolin
Caveolin 1 - metabolism
Caveolin-1
Cell Membrane - metabolism
Cell membranes
CHO Cells
Cholesterol
Cricetulus
Cytoskeleton
Desensitization
Endocytosis
Energy transfer
Experiments
Fluorescence
Fluorescence resonance energy transfer
Fluorescence Resonance Energy Transfer - methods
Localization
Membranes
Microscopy
Microtubules
Microtubules - metabolism
Nanotechnology
Pain perception
Proteins
Selective binding
Spectrum analysis
Transient receptor potential proteins
TRPV Cation Channels - metabolism
Variation
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Summary:Transient Receptor Potential Vanilloid 1 (TRPV1) is a non-selective cation channel that integrates several stimuli into nociception and neurogenic inflammation. Here we investigated the subtle TRPV1 interplay with candidate membrane partners in live cells by a combination of spatio-temporal fluctuation techniques and fluorescence resonance energy transfer (FRET) imaging. We show that TRPV1 is split into three populations with fairly different molecular properties: one binding to caveolin-1 and confined into caveolar structures, one actively guided by microtubules through selective binding, and one which diffuses freely and is not directly implicated in regulating receptor functionality. The emergence of caveolin-1 as a new interactor of TRPV1 evokes caveolar endocytosis as the main desensitization pathway of TRPV1 receptor, while microtubule binding agrees with previous data suggesting the receptor stabilization in functional form by these cytoskeletal components. Our results shed light on the hitherto unknown relationships between spatial organization and TRPV1 function in live-cell membranes.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0116900