Direct detection of fungal siderophores on bats with white-nose syndrome via fluorescence microscopy-guided ambient ionization mass spectrometry

White-nose syndrome (WNS) caused by the pathogenic fungus Pseudogymnoascus destructans is decimating the populations of several hibernating North American bat species. Little is known about the molecular interplay between pathogen and host in this disease. Fluorescence microscopy ambient ionization...

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Bibliographic Details
Published in:PloS one 2015-03, Vol.10 (3), p.e0119668-e0119668
Main Authors: Mascuch, Samantha J, Moree, Wilna J, Hsu, Cheng-Chih, Turner, Gregory G, Cheng, Tina L, Blehert, David S, Kilpatrick, A Marm, Frick, Winifred F, Meehan, Michael J, Dorrestein, Pieter C, Gerwick, Lena
Format: Article
Language:English
Subjects:
Animal diseases
Animals
Ascomycota - isolation & purification
Ascomycota - metabolism
Ascomycota - pathogenicity
Aspergillus fumigatus
Atmospheric ionization
Bats
Biochemistry
Biotechnology
Caretta caretta
Chiroptera
Chiroptera - microbiology
Evolutionary biology
Fluorescence
Fluorescence microscopy
Fungi
Infections
Ionization
Iron
Mass spectrometry
Mass spectroscopy
Microorganisms
Microscopy
Microscopy, Fluorescence
Myotis
Myotis lucifugus
Nose
Oceanography
Pharmaceutical sciences
Pharmacy
Scientific imaging
Siderophores
Siderophores - chemistry
Siderophores - isolation & purification
Spectrometry, Mass, Secondary Ion
Spectroscopy
White-nose syndrome
Wings
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Summary:White-nose syndrome (WNS) caused by the pathogenic fungus Pseudogymnoascus destructans is decimating the populations of several hibernating North American bat species. Little is known about the molecular interplay between pathogen and host in this disease. Fluorescence microscopy ambient ionization mass spectrometry was used to generate metabolic profiles from the wings of both healthy and diseased bats of the genus Myotis. Fungal siderophores, molecules that scavenge iron from the environment, were detected on the wings of bats with WNS, but not on healthy bats. This work is among the first examples in which microbial molecules are directly detected from an infected host and highlights the ability of atmospheric ionization methodologies to provide direct molecular insight into infection.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0119668