Comparison of normal saline, hypertonic saline albumin and terlipressin plus hypertonic saline albumin in an infant animal model of hypovolemic shock

• Published in: PloS one 2015-03, Vol.10 (3), p.e0121678-e0121678
• Main Authors: Urbano, Javier, González, Rafael, López, Jorge, Solana, María J, Bellón, José M, Botrán, Marta, García, Ana, Fernández, Sarah N, López-Herce, Jesús
• Format: Article
• Language: English
• Subjects: Albumin; Albumins - pharmacology; Albumins - therapeutic use; Animal models; Animals; Animals, Newborn; Bleeding; Blood; Blood flow; Blood pressure; Blood Pressure - drug effects; Brain; Carbon Dioxide - metabolism; Cerebral blood flow; Children & youth; Childrens health; Comparative analysis; Disease Models, Animal; Drug delivery systems; Fluid; Fluids; Heart diseases; Heart rate; Heart Rate - drug effects; Hemodynamics; Hemodynamics - drug effects; Hemorrhage; Hemorrhage - pathology; Hemorrhage - physiopathology; Hypovolemia - drug therapy; Hypovolemia - physiopathology; Intensive care; Lactates; Lactic acid; Lypressin - analogs & derivatives; Lypressin - pharmacology; Lypressin - therapeutic use; Maternal & child health; Mathematical models; Medical research; Multiple organ dysfunction syndrome; Oxygen - blood; Pediatrics; Perfusion; Peripheral blood; Pituitary hormones; Randomization; Resuscitation; Rodents; Saline Solution, Hypertonic - pharmacology; Saline Solution, Hypertonic - therapeutic use; Saturation; Shock; Sodium Chloride - pharmacology; Sodium Chloride - therapeutic use; Stroke; Stroke volume; Stroke Volume - drug effects; Sus scrofa; Swine; Vasopressin
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0121678

In series of cases and animal models suffering hemorrhagic shock, the use of vasopressors has shown potential benefits regarding hemodynamics and tissue perfusion. Terlipressin is an analogue of vasopressin with a longer half-life that can be administered by bolus injection. We have previously observed that hypertonic albumin improves resuscitation following controlled hemorrhage in piglets. The aim of the present study was to analyze whether the treatment with the combination of terlipressin and hypertonic albumin can produce better hemodynamic and tissular perfusion parameters than normal saline or hypertonic albumin alone at early stages of hemorrhagic shock in an infant animal model. Experimental, randomized animal study including 39 2-to-3-month-old piglets. Thirty minutes after controlled 30 ml/kg bleed, pigs were randomized to receive either normal saline (NS) 30 ml/kg (n = 13), 5% albumin plus 3% hypertonic saline (AHS) 15 ml/kg (n = 13) or single bolus of terlipressin 15 μg/kg i.v. plus 5% albumin plus 3% hypertonic saline 15 ml/kg (TAHS) (n = 13) over 30 minutes. Global hemodynamic and tissular perfusion parameters were compared. After controlled bleed a significant decrease of blood pressure, cardiac index, central venous saturation, carotid and peripheral blood flow, brain saturation and an increase of heart rate, gastric PCO2 and lactate was observed. After treatment no significant differences in most hemodynamic (cardiac index, mean arterial pressure) and perfusion parameters (lactate, gastric PCO2, brain saturation, cutaneous blood flow) were observed between the three therapeutic groups. AHS and TAHS produced higher increase in stroke volume index and carotid blood flow than NS. In this pediatric animal model of hypovolemic shock, albumin plus hypertonic saline with or without terlipressin achieved similar hemodynamics and perfusion parameters than twice the volume of NS. Addition of terlipressin did not produce better results than AHS.