Multimodal holographic microscopy: distinction between apoptosis and oncosis

Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However,...

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Bibliographic Details
Published in:PloS one 2015-03, Vol.10 (3), p.e0121674-e0121674
Main Authors: Balvan, Jan, Krizova, Aneta, Gumulec, Jaromir, Raudenska, Martina, Sladek, Zbysek, Sedlackova, Miroslava, Babula, Petr, Sztalmachova, Marketa, Kizek, Rene, Chmelik, Radim, Masarik, Michal
Format: Article
Language:English
Subjects:
Annexin V
Apoptosis
Autophagy
Cancer
Cell cycle
Cell death
Cell Line, Tumor
Cell Survival
Cytology
Cytometry
Fluorescence
Gangrene
Holography - methods
Humans
Iodides
Medicine
Microscopy
Microscopy, Fluorescence - methods
Morphology
Mortality
Multimodal Imaging - methods
Necrosis
Phenotype
Phenotypes
Physiology
Propidium iodide
Time Factors
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Summary:Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection - "Multimodal holographic microscopy (MHM)". The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI- phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0121674