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Abnormal development of tapetum and microspores induced by chemical hybridization agent SQ-1 in wheat
Chemical hybridization agent (CHA)-induced male sterility is an important tool in crop heterosis. To demonstrate that CHA-SQ-1-induced male sterility is associated with abnormal tapetal and microspore development, the cytology of CHA-SQ-1-treated plant anthers at various developmental stages was stu...
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Published in: | PloS one 2015-03, Vol.10 (3), p.e0119557-e0119557 |
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description | Chemical hybridization agent (CHA)-induced male sterility is an important tool in crop heterosis. To demonstrate that CHA-SQ-1-induced male sterility is associated with abnormal tapetal and microspore development, the cytology of CHA-SQ-1-treated plant anthers at various developmental stages was studied by light microscopy, scanning and transmission electron microscopy, in situ terminal deoxynucleotidyl transferasemediated dUTP nick end-labelling (TUNEL) assay and DAPI staining. The results indicated that the SQ-1-treated plants underwent premature tapetal programmed cell death (PCD), which was initiated at the early-uninucleate stage of microspore development and continued until the tapetal cells were completely degraded; the process of microspore development was then blocked. Microspores with low-viability (fluorescein diacetate staining) were aborted. The study suggests that premature tapetal PCD is the main cause of pollen abortion. Furthermore, it determines the starting period and a key factor in CHA-SQ-1-induced male sterility at the cell level, and provides cytological evidence to further study the mechanism between PCD and male sterility. |
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To demonstrate that CHA-SQ-1-induced male sterility is associated with abnormal tapetal and microspore development, the cytology of CHA-SQ-1-treated plant anthers at various developmental stages was studied by light microscopy, scanning and transmission electron microscopy, in situ terminal deoxynucleotidyl transferasemediated dUTP nick end-labelling (TUNEL) assay and DAPI staining. The results indicated that the SQ-1-treated plants underwent premature tapetal programmed cell death (PCD), which was initiated at the early-uninucleate stage of microspore development and continued until the tapetal cells were completely degraded; the process of microspore development was then blocked. Microspores with low-viability (fluorescein diacetate staining) were aborted. The study suggests that premature tapetal PCD is the main cause of pollen abortion. Furthermore, it determines the starting period and a key factor in CHA-SQ-1-induced male sterility at the cell level, and provides cytological evidence to further study the mechanism between PCD and male sterility.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0119557</identifier><identifier>PMID: 25803723</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abortion ; Agricultural biotechnology ; Agronomy ; Anthers ; Apoptosis ; Apoptosis - drug effects ; Cell death ; Cytology ; Developmental stages ; Education ; Electron microscopy ; Engineering research ; Fertility ; Flowers - cytology ; Flowers - drug effects ; Fluorescein ; Fluorescein diacetate ; Gametogenesis, Plant - drug effects ; Gametogenesis, Plant - genetics ; Gene expression ; Heterosis ; Hybrid Vigor ; Hybridization ; In Situ Nick-End Labeling ; Labeling ; Labelling ; Laboratories ; Light microscopy ; Male sterility ; Microscopy ; Microspores ; Molecular biology ; Plant Infertility - drug effects ; Plant reproduction ; Pollen ; Pollen - cytology ; Pollen - drug effects ; Pyridazines - pharmacology ; Rice ; Scanning electron microscopy ; Seeds ; Staining ; Sterility ; Tobacco ; Transcription factors ; Transmission electron microscopy ; Triticum - drug effects ; Triticum - genetics ; Triticum - growth & development ; Viability ; Wheat</subject><ispartof>PloS one, 2015-03, Vol.10 (3), p.e0119557-e0119557</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Wang et al 2015 Wang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c758t-46ae1823d622428937e5605c39bb5287d96205eee43e37dc72a9592c77d876223</citedby><cites>FETCH-LOGICAL-c758t-46ae1823d622428937e5605c39bb5287d96205eee43e37dc72a9592c77d876223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1666309772/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1666309772?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25803723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Niedz, Randall P.</contributor><creatorcontrib>Wang, Shuping</creatorcontrib><creatorcontrib>Zhang, Gaisheng</creatorcontrib><creatorcontrib>Song, Qilu</creatorcontrib><creatorcontrib>Zhang, Yingxin</creatorcontrib><creatorcontrib>Li, Zheng</creatorcontrib><creatorcontrib>Guo, Jialin</creatorcontrib><creatorcontrib>Niu, Na</creatorcontrib><creatorcontrib>Ma, Shoucai</creatorcontrib><creatorcontrib>Wang, Junwei</creatorcontrib><title>Abnormal development of tapetum and microspores induced by chemical hybridization agent SQ-1 in wheat</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Chemical hybridization agent (CHA)-induced male sterility is an important tool in crop heterosis. To demonstrate that CHA-SQ-1-induced male sterility is associated with abnormal tapetal and microspore development, the cytology of CHA-SQ-1-treated plant anthers at various developmental stages was studied by light microscopy, scanning and transmission electron microscopy, in situ terminal deoxynucleotidyl transferasemediated dUTP nick end-labelling (TUNEL) assay and DAPI staining. The results indicated that the SQ-1-treated plants underwent premature tapetal programmed cell death (PCD), which was initiated at the early-uninucleate stage of microspore development and continued until the tapetal cells were completely degraded; the process of microspore development was then blocked. Microspores with low-viability (fluorescein diacetate staining) were aborted. The study suggests that premature tapetal PCD is the main cause of pollen abortion. Furthermore, it determines the starting period and a key factor in CHA-SQ-1-induced male sterility at the cell level, and provides cytological evidence to further study the mechanism between PCD and male sterility.</description><subject>Abortion</subject><subject>Agricultural biotechnology</subject><subject>Agronomy</subject><subject>Anthers</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Cell death</subject><subject>Cytology</subject><subject>Developmental stages</subject><subject>Education</subject><subject>Electron microscopy</subject><subject>Engineering research</subject><subject>Fertility</subject><subject>Flowers - cytology</subject><subject>Flowers - drug effects</subject><subject>Fluorescein</subject><subject>Fluorescein diacetate</subject><subject>Gametogenesis, Plant - drug effects</subject><subject>Gametogenesis, Plant - genetics</subject><subject>Gene expression</subject><subject>Heterosis</subject><subject>Hybrid Vigor</subject><subject>Hybridization</subject><subject>In Situ Nick-End Labeling</subject><subject>Labeling</subject><subject>Labelling</subject><subject>Laboratories</subject><subject>Light microscopy</subject><subject>Male sterility</subject><subject>Microscopy</subject><subject>Microspores</subject><subject>Molecular biology</subject><subject>Plant Infertility - drug effects</subject><subject>Plant reproduction</subject><subject>Pollen</subject><subject>Pollen - cytology</subject><subject>Pollen - drug effects</subject><subject>Pyridazines - pharmacology</subject><subject>Rice</subject><subject>Scanning electron microscopy</subject><subject>Seeds</subject><subject>Staining</subject><subject>Sterility</subject><subject>Tobacco</subject><subject>Transcription factors</subject><subject>Transmission electron microscopy</subject><subject>Triticum - drug effects</subject><subject>Triticum - genetics</subject><subject>Triticum - growth & development</subject><subject>Viability</subject><subject>Wheat</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QLgujFjPlokvZGGBY_BhYWXfU2pMnpTIa26Sbt6vjrTZ3uMpW9kF60JM_7pufkPUnyHKMlpgK_27nBt6pedq6FJcK4YEw8SE5xQcmCE0QfHn2fJE9C2CHEaM754-SEsBxRQehpAquydb5RdWrgBmrXNdD2qavSXnXQD02qWpM2VnsXOuchpLY1gwaTlvtUbyHuROl2X3pr7G_VW9emajNaXH1Z4AinP7eg-qfJo0rVAZ5N77Pk-8cP384_Ly4uP63PVxcLLVjeLzKuAOeEGk5IRvKCCmAcMU2LsmQkF6aIxTAAyChQYbQgqmAF0UKYXEQNPUteHny72gU5dShIzDmnqBBiJNYHwji1k523jfJ76ZSVfxec30jle6trkJhykYsCI2JEZjRTVVkC1qzEHFUlyqLX--m0oWzA6Fi2V_XMdL7T2q3cuBuZjb3PeDR4Mxl4dz1A6GVjg4a6Vi244fDfRZ5TOqKv_kHvr26iNioWYNvKxXP1aCpXWeyPQJyKSC3voeJjxvuMcapsXJ8J3s4EkenhV79RQwhyffX1_9nLH3P29REbg1L32-DqYYxRmIPZARxzGDxUd03GSI7TcNsNOU6DnKYhyl4cX9Cd6Db-9A_OlQNu</recordid><startdate>20150324</startdate><enddate>20150324</enddate><creator>Wang, Shuping</creator><creator>Zhang, Gaisheng</creator><creator>Song, Qilu</creator><creator>Zhang, Yingxin</creator><creator>Li, Zheng</creator><creator>Guo, Jialin</creator><creator>Niu, Na</creator><creator>Ma, Shoucai</creator><creator>Wang, Junwei</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150324</creationdate><title>Abnormal development of tapetum and microspores induced by chemical hybridization agent SQ-1 in wheat</title><author>Wang, Shuping ; Zhang, Gaisheng ; Song, Qilu ; Zhang, Yingxin ; Li, Zheng ; Guo, Jialin ; Niu, Na ; Ma, Shoucai ; Wang, Junwei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c758t-46ae1823d622428937e5605c39bb5287d96205eee43e37dc72a9592c77d876223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Abortion</topic><topic>Agricultural biotechnology</topic><topic>Agronomy</topic><topic>Anthers</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Cell death</topic><topic>Cytology</topic><topic>Developmental stages</topic><topic>Education</topic><topic>Electron microscopy</topic><topic>Engineering research</topic><topic>Fertility</topic><topic>Flowers - cytology</topic><topic>Flowers - drug effects</topic><topic>Fluorescein</topic><topic>Fluorescein diacetate</topic><topic>Gametogenesis, Plant - drug effects</topic><topic>Gametogenesis, Plant - genetics</topic><topic>Gene expression</topic><topic>Heterosis</topic><topic>Hybrid Vigor</topic><topic>Hybridization</topic><topic>In Situ Nick-End Labeling</topic><topic>Labeling</topic><topic>Labelling</topic><topic>Laboratories</topic><topic>Light microscopy</topic><topic>Male sterility</topic><topic>Microscopy</topic><topic>Microspores</topic><topic>Molecular biology</topic><topic>Plant Infertility - drug effects</topic><topic>Plant reproduction</topic><topic>Pollen</topic><topic>Pollen - cytology</topic><topic>Pollen - drug effects</topic><topic>Pyridazines - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Shuping</au><au>Zhang, Gaisheng</au><au>Song, Qilu</au><au>Zhang, Yingxin</au><au>Li, Zheng</au><au>Guo, Jialin</au><au>Niu, Na</au><au>Ma, Shoucai</au><au>Wang, Junwei</au><au>Niedz, Randall P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abnormal development of tapetum and microspores induced by chemical hybridization agent SQ-1 in wheat</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-03-24</date><risdate>2015</risdate><volume>10</volume><issue>3</issue><spage>e0119557</spage><epage>e0119557</epage><pages>e0119557-e0119557</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Chemical hybridization agent (CHA)-induced male sterility is an important tool in crop heterosis. To demonstrate that CHA-SQ-1-induced male sterility is associated with abnormal tapetal and microspore development, the cytology of CHA-SQ-1-treated plant anthers at various developmental stages was studied by light microscopy, scanning and transmission electron microscopy, in situ terminal deoxynucleotidyl transferasemediated dUTP nick end-labelling (TUNEL) assay and DAPI staining. The results indicated that the SQ-1-treated plants underwent premature tapetal programmed cell death (PCD), which was initiated at the early-uninucleate stage of microspore development and continued until the tapetal cells were completely degraded; the process of microspore development was then blocked. Microspores with low-viability (fluorescein diacetate staining) were aborted. The study suggests that premature tapetal PCD is the main cause of pollen abortion. Furthermore, it determines the starting period and a key factor in CHA-SQ-1-induced male sterility at the cell level, and provides cytological evidence to further study the mechanism between PCD and male sterility.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>25803723</pmid><doi>10.1371/journal.pone.0119557</doi><oa>free_for_read</oa></addata></record> |
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subjects | Abortion Agricultural biotechnology Agronomy Anthers Apoptosis Apoptosis - drug effects Cell death Cytology Developmental stages Education Electron microscopy Engineering research Fertility Flowers - cytology Flowers - drug effects Fluorescein Fluorescein diacetate Gametogenesis, Plant - drug effects Gametogenesis, Plant - genetics Gene expression Heterosis Hybrid Vigor Hybridization In Situ Nick-End Labeling Labeling Labelling Laboratories Light microscopy Male sterility Microscopy Microspores Molecular biology Plant Infertility - drug effects Plant reproduction Pollen Pollen - cytology Pollen - drug effects Pyridazines - pharmacology Rice Scanning electron microscopy Seeds Staining Sterility Tobacco Transcription factors Transmission electron microscopy Triticum - drug effects Triticum - genetics Triticum - growth & development Viability Wheat |
title | Abnormal development of tapetum and microspores induced by chemical hybridization agent SQ-1 in wheat |
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