Ionizing radiation-induced adaptive response in fibroblasts under both monolayer and 3-dimensional conditions

• Published in: PloS one 2015-03, Vol.10 (3), p.e0121289-e0121289
• Main Authors: Zhao, Yinlong, Zhong, Rui, Sun, Liguang, Jia, Jie, Ma, Shumei, Liu, Xiaodong
• Format: Article
• Language: English
• Subjects: Adaptation, Biological - radiation effects; Analysis; Apoptosis; Cell culture; Cell cycle; Cell death; Cell Death - radiation effects; Cells, Cultured; Collagen; Cyclin-dependent kinase inhibitor p21; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Deoxyribonucleic acid; DNA; DNA damage; DNA Damage - radiation effects; DNA repair; Dose-Response Relationship, Radiation; Drug dosages; Fibroblasts; Fibroblasts - metabolism; Fibroblasts - radiation effects; Histones - metabolism; Hospitals; Humans; Ionizing radiation; Kinases; Laboratories; Lymphocytes; Monolayers; Monomolecular films; Mutagenesis; p53 Protein; Priming; Protein expression; Proteins; Public health; Radiation; Radiation damage; Radiation dosage; Radiation effects; Radiation, Ionizing; Staining; Texture; Time dependence; Tumor proteins; Tumor Suppressor Protein p53 - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0121289

To observe the adaptive response (AR) induced by ionizing radiation in human fibroblasts under monolayer and 3-dimensional (3-D) condition. Three kinds of fibroblasts were cultured under both monolayer and 3-D condition. Immunofluorescent staining was used to detect the γ-H2AX foci and the morphological texture. Trypan blue staining was used to detect the cell death. Western blot was used to detect the expressions of γ-H2AX, p53 and CDKN1A/p21 (p21). We found that DNA damage increased in a dose-dependent and time-dependent manner after high doses of radiation. When cells were pretreated with a priming low dose of radiation followed by high dose radiation, DNA damage was attenuated under both monolayer and 3-D condition, and the adaptive response (AR) was induced. Additionally, the morphology of cells under monolayer and 3-D conditions were different, and radiation also induced AR according to morphological texture analysis. Priming low dose radiation induced AR both under monolayer and 3-D condition. Interestingly, 3-D microenvironment made cells more sensitive to radiation. The expression of p53 and p21 was changed and indicated that they might participate in the regulation of AR.