Fusarium oxysporum triggers tissue-specific transcriptional reprogramming in Arabidopsis thaliana

Some of the most devastating agricultural diseases are caused by root-infecting pathogens, yet the majority of studies on these interactions to date have focused on the host responses of aerial tissues rather than those belowground. Fusarium oxysporum is a root-infecting pathogen that causes wilt di...

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Bibliographic Details
Published in:PloS one 2015-04, Vol.10 (4), p.e0121902-e0121902
Main Authors: Lyons, Rebecca, Stiller, Jiri, Powell, Jonathan, Rusu, Anca, Manners, John M, Kazan, Kemal
Format: Article
Language:English
Subjects:
Abscisic acid
Analysis
Arabidopsis
Arabidopsis - microbiology
Arabidopsis thaliana
Biosynthesis
Defensins
Fungal Proteins - biosynthesis
Fusarium
Fusarium - metabolism
Fusarium oxysporum
Gene expression
Gene Expression Regulation, Fungal - physiology
Genes
Genetic aspects
Jasmonic acid
Leaves
Lectins
Mannose
Pathogens
Plant diseases
Plant Diseases - microbiology
Plant Leaves - microbiology
Plant Roots - microbiology
Plant species
Plant tissues
Plants (botany)
Regulators
Ribonucleic acid
RNA
Senescence
Signaling
Transcription
Transcription (Genetics)
Wilt
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Summary:Some of the most devastating agricultural diseases are caused by root-infecting pathogens, yet the majority of studies on these interactions to date have focused on the host responses of aerial tissues rather than those belowground. Fusarium oxysporum is a root-infecting pathogen that causes wilt disease on several plant species including Arabidopsis thaliana. To investigate and compare transcriptional changes triggered by F. oxysporum in different Arabidopsis tissues, we infected soil-grown plants with F. oxysporum and subjected root and leaf tissue harvested at early and late timepoints to RNA-seq analyses. At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation. Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue. We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum. In the leaves, defensins and genes associated with the response to auxin, cold and senescence were strongly regulated while jasmonate biosynthesis and signalling genes were induced throughout the plant.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0121902