Correction: Antiapoptotic factor humanin is expressed in normal and tumoral pituitary cells and protects them from TNF-α-induced apoptosis

• Published in: PloS one 2015-04, Vol.10 (4), p.e0124589-e0124589
• Main Authors: Gottardo, María Florencia, Jaita, Gabriela, Magri, María Laura, Zárate, Sandra, Ayala, Mariela Moreno, Ferraris, Jimena, Eijo, Guadalupe, Pisera, Daniel, Candolfi, Marianela, Seilicovich, Adriana
• Format: Article
• Language: English
• Subjects: 17β-Estradiol; Apoptosis; Experiments; Rodents
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0124589

The eighth sentence of the Abstract should read: HN (0.5 μM) per se did not modify basal apoptosis of anterior pituitary cells but completely blocked the proapoptotic effect of TNF-α in total anterior pituitary cells, lactotropes and somatotropes from both female and male rats. Cultured anterior pituitary cells from OVX rats were incubated with 17β-estradiol (E2, 10-9 M) for 24 h and then with HN 0.25 μM (A), 0.5 μM (B), or 1 μM (C) for 2 h before adding TNF-α (50 ng/ml) for an additional 24 h. Apoptosis was assessed by the TUNEL method. Cultured anterior pituitary cells from GNX male rats were incubated with dihydrotestosterone (DHT, 10-8 M) for 24 h and then with HN (0.5 μM) for 2 h before adding TNF-α (50 ng/ml) for an additional 24 h. Apoptosis was assessed by the TUNEL method and lactotropes and somatotropes detected by immunofluorescence.