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Matrix-assisted laser desorption/ionization time of flight mass-spectrometry (MALDI-TOF MS) based typing of extended-spectrum β-lactamase producing E. coli--a novel tool for real-time outbreak investigation

Epidemiologically linked clusters are confirmed by typing strains with molecular typing such as pulsed-field gel electrophoresis (PFGE). We compared six extended-spectrum β-lactamase producing E. coli of a PFGE-related cluster with Matrix-assisted laser desorption/ionization-time of flight mass-spec...

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Bibliographic Details
Published in:PloS one 2015-04, Vol.10 (4), p.e0120624-e0120624
Main Authors: Egli, Adrian, Tschudin-Sutter, Sarah, Oberle, Michael, Goldenberger, Daniel, Frei, Reno, Widmer, Andreas F
Format: Article
Language:English
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Summary:Epidemiologically linked clusters are confirmed by typing strains with molecular typing such as pulsed-field gel electrophoresis (PFGE). We compared six extended-spectrum β-lactamase producing E. coli of a PFGE-related cluster with Matrix-assisted laser desorption/ionization-time of flight mass-spectrometry based typing that confirmed relatedness faster and more cost-effective, but as reliable as PFGE.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0120624