Digital gene expression analysis of Microsporum canis exposed to berberine chloride

Berberine, a natural isoquinoline alkaloid of many medicinal herbs, has an active function against a variety of microbial infections including Microsporum canis (M. canis). However, the underlying mechanisms are poorly understood. To study the effect of berberine chloride on M. canis infection, a Di...

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Bibliographic Details
Published in:PloS one 2015-04, Vol.10 (4), p.e0124265-e0124265
Main Authors: Xiao, Chen-Wen, Ji, Quan-An, Wei, Qiang, Liu, Yan, Pan, Li-Jun, Bao, Guo-Lian
Format: Article
Language:English
Subjects:
Analysis
Apoptosis
Arabidopsis
Berberine
Berberine - pharmacology
Biosynthesis
Breeding of animals
Calcium
Chlorides
Down-Regulation - drug effects
Drug dosages
Enrichment
Fungi
Gene expression
Gene Expression Profiling
Gene sequencing
Genes
Genomes
Glycerol
Health aspects
Herbal medicine
Infections
Ketoglutaric acid
Kinases
Medicinal herbs
Medicine
Microorganisms
Microsporum - genetics
Microsporum - metabolism
Phellodendron amurense
Piperidine
Plants, Medicinal - chemistry
Plants, Medicinal - metabolism
Protein binding
Protein folding
Pulmonary arteries
Real-Time Polymerase Chain Reaction
Science
Signal transduction
Signal Transduction - drug effects
Signaling
Studies
Transcriptome
Tropane
Up-Regulation - drug effects
Valine
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Summary:Berberine, a natural isoquinoline alkaloid of many medicinal herbs, has an active function against a variety of microbial infections including Microsporum canis (M. canis). However, the underlying mechanisms are poorly understood. To study the effect of berberine chloride on M. canis infection, a Digital Gene Expression (DGE) tag profiling was constructed and a transcriptome analysis of the M. canis cellular responses upon berberine treatment was performed. Illumina/Hisseq sequencing technique was used to generate the data of gene expression profile, and the following enrichment analysis of Gene Ontology (GO) and Pathway function were conducted based on the data of transcriptome. The results of DGE showed that there were 8476945, 14256722, 7708575, 5669955, 6565513 and 9303468 tags respectively, which was obtained from M. canis incubated with berberine or control DMSO. 8,783 genes were totally mapped, and 1,890 genes have shown significant changes between the two groups. 1,030 genes were up-regulated and 860 genes were down-regulated (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0124265