Altered metabolic and stemness capacity of adipose tissue-derived stem cells from obese mouse and human

Adipose stem cells (ASCs) are an appealing source of cells for therapeutic intervention; however, the environment from which ASCs are isolated may impact their usefulness. Using a range of functional assays, we have evaluated whether ASCs isolated from an obese environment are comparable to cells fr...

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Bibliographic Details
Published in:PloS one 2015-04, Vol.10 (4), p.e0123397-e0123397
Main Authors: Pérez, Laura M, Bernal, Aurora, de Lucas, Beatriz, San Martin, Nuria, Mastrangelo, Annalaura, García, Antonia, Barbas, Coral, Gálvez, Beatriz G
Format: Article
Language:English
Subjects:
Adipocytes
Adipocytes - cytology
Adipose tissue
Adipose Tissue - pathology
Adult
Aging
Analysis
Animals
Apoptosis
Base Sequence
Body fat
Body Mass Index
Cell growth
Cell Proliferation
Cell self-renewal
Cell Survival
Deoxyribonucleic acid
Diabetes
DNA
DNA - analysis
Ethics
Fatty Acids - chemistry
Female
Gastrointestinal surgery
Health aspects
Homeostasis
Humans
Hypoxia
Insulin resistance
Lactic Acid - chemistry
Metabolism
Mice
Mice, Inbred C57BL
Middle Aged
Mitochondria
Mitochondria - metabolism
Molecular Sequence Data
Obesity
Obesity - metabolism
Reactive Oxygen Species - metabolism
Stem cell transplantation
Stem cells
Stem Cells - cytology
Studies
Substrates
Telomerase
Telomere - ultrastructure
Viability
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Summary:Adipose stem cells (ASCs) are an appealing source of cells for therapeutic intervention; however, the environment from which ASCs are isolated may impact their usefulness. Using a range of functional assays, we have evaluated whether ASCs isolated from an obese environment are comparable to cells from non-obese adipose tissue. Results showed that ASCs isolated from obese tissue have a reduced proliferative ability and a loss of viability together with changes in telomerase activity and DNA telomere length, suggesting a decreased self-renewal capacity. Metabolic analysis demonstrated that mitochondrial content and function was impaired in obese-derived ASCs resulting in changes in favored oxidative substrates. These findings highlight the impact of obesity on adult stem properties. Hence, caution should be exercised when considering the source of ASCs for cellular therapies since their therapeutic potential may be impaired.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0123397