Genome-wide association study of serum minerals levels in children of different ethnic background

• Published in: PloS one 2015-04, Vol.10 (4), p.e0123499-e0123499
• Main Authors: Chang, Xiao, Li, Jin, Guo, Yiran, Wei, Zhi, Mentch, Frank D, Hou, Cuiping, Zhao, Yan, Qiu, Haijun, Kim, Cecilia, Sleiman, Patrick M A, Hakonarson, Hakon
• Format: Article
• Language: English
• Subjects: African Americans; Association analysis; Biological products; Calcium; Calcium (blood); Calcium chloride; Calcium-sensing receptors; Child; Children; Chromosome 1; Chromosome 3; Diet; Electronic health records; Electronic medical records; European Continental Ancestry Group; GATA-3 protein; Genome-wide association studies; Genome-Wide Association Study; Genomes; Genomics; Humans; Loci; Magnesium; Minerals; Minerals - blood; Mucins; Phosphorus; Potassium; Sodium
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0123499

Calcium, magnesium, potassium, sodium, chloride and phosphorus are the major dietary minerals involved in various biological functions and are commonly measured in the blood serum. Sufficient mineral intake is especially important for children due to their rapid growth. Currently, the genetic mechanisms influencing serum mineral levels are poorly understood, especially for children. We carried out a genome-wide association (GWA) study on 5,602 European-American children and 4,706 African-American children who had mineral measures available in their electronic medical records (EMR). While no locus met the criteria for genome-wide significant association, our results demonstrated a nominal association of total serum calcium levels with a missense variant in the calcium -sensing receptor (CASR) gene on 3q13 (rs1801725, P = 1.96 × 10(-3)) in the African-American pediatric cohort, a locus previously reported in Caucasians. We also confirmed the association result in our pediatric European-American cohort (P = 1.38 × 10(-4)). We further replicated two other loci associated with serum calcium levels in the European-American cohort (rs780094, GCKR, P = 4.26 × 10(-3); rs10491003, GATA3, P = 0.02). In addition, we replicated a previously reported locus on 1q21, demonstrating association of serum magnesium levels with MUC1 (rs4072037, P = 2.04 × 10(-6)). Moreover, in an extended gene-based association analysis we uncovered evidence for association of calcium levels with the previously reported gene locus DGKD in both European-American children and African-American children. Taken together, our results support a role for CASR and DGKD mediated calcium regulation in both African-American and European-American children, and corroborate the association of calcium levels with GCKR and GATA3, and the association of magnesium levels with MUC1 in the European-American children.