Action spectrum for photobleaching of human lenses by short wavelength visible irradiation

Cataract is the world-leading cause of blindness. In search for a new treatment of cataract we have found that the yellow discolouration of aged human lenses can be photobleached using a non-invasive, infra-red, femtosecond laser treatment. These results were presented in an earlier PlosOne publicat...

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Published in:PloS one 2015-04, Vol.10 (4), p.e0123732-e0123732
Main Authors: Kessel, Line, Larsen, Michael
Format: Article
Language:English
Subjects:
Absorbents
Action Spectrum
Adult
Aged
Aged, 80 and over
Aging
Bleaches
Bleaching
Blindness
Cataract - therapy
Cataracts
Continuous wave lasers
Discoloration
Femtosecond
Femtosecond lasers
Humans
In vitro methods and tests
Irradiation
Lasers
Lens, Crystalline - radiation effects
Lenses
Light
Middle Aged
Photobleaching
Photography
Phototherapy
Wavelengths
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description Cataract is the world-leading cause of blindness. In search for a new treatment of cataract we have found that the yellow discolouration of aged human lenses can be photobleached using a non-invasive, infra-red, femtosecond laser treatment. These results were presented in an earlier PlosOne publication. The objective of the study was to characterize the single-photon photobleaching action spectrum of the aged human lens in vitro. Ninety-one human donor lenses were irradiated with continuous wave laser light at 375, 405, 420, 445, 457 or 473 nm. Photobleaching was monitored by photography and transmission measurements. The action spectrum peaked at 420 nm followed by, in order of decreasing effect, 445, 457, 473, 405 and 375 nm. Younger and less absorbent lenses showed smaller changes than older and more absorbent lenses. There was a dose-dependent increase in lens transmission with increasing laser irradiation. For a 75 year old lens an effect corresponding to elimination of 15 years or more of optical ageing was obtained. This study of the spectral characteristics and intensity needed to bleach the human lens with single-photon laser effects found an action-spectrum peak at 420 nm tailing gradually off toward longer wavelengths and more steeply toward shorter wavelengths. The results may be used to guide experiments with two-photon bleaching.
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subjects Absorbents
Action Spectrum
Adult
Aged
Aged, 80 and over
Aging
Bleaches
Bleaching
Blindness
Cataract - therapy
Cataracts
Continuous wave lasers
Discoloration
Femtosecond
Femtosecond lasers
Humans
In vitro methods and tests
Irradiation
Lasers
Lens, Crystalline - radiation effects
Lenses
Light
Middle Aged
Photobleaching
Photography
Phototherapy
Wavelengths
title Action spectrum for photobleaching of human lenses by short wavelength visible irradiation
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