Inhibitors of eicosanoid biosynthesis influencing the transcripts level of sHSP21.4 gene induced by pathogen infections, in Antheraea pernyi

• Published in: PloS one 2015-04, Vol.10 (4), p.e0121296-e0121296
• Main Authors: Zhang, Congfen, Dai, Lishang, Wang, Lei, Qian, Cen, Wei, Guoqing, Li, Jun, Zhu, Baojian, Liu, Chaoliang
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Antheraea pernyi; Aromatic-L-amino-acid decarboxylase; Bacterial infections; Beauveria - physiology; Beauveria bassiana; Bioinformatics; Biosynthesis; Cell cycle; Ceratitis capitata; Cloning, Molecular; Dexamethasone - pharmacology; Dihydroxyphenylalanine; Double-stranded RNA; E coli; Eicosanoic Acids - metabolism; Eicosanoids; Escherichia coli - physiology; Fat body; Fungi; Gene expression; Gene Expression Regulation - drug effects; Health aspects; Heat shock proteins; Heat-Shock Proteins, Small - chemistry; Heat-Shock Proteins, Small - genetics; Heat-Shock Proteins, Small - metabolism; Immune response; Immune system; Immunity; Indomethacin - pharmacology; Infections; Inhibitors; Insect Proteins - chemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Insects; Laboratories; Lepidoptera; Life sciences; Lipid Metabolism - drug effects; Lysozyme; Masoprocol - pharmacology; Microorganisms; Midgut; Moths - genetics; Moths - immunology; Moths - microbiology; Nucleopolyhedroviruses - physiology; Pathogens; Phylogenetics; Phylogeny; Protease inhibitors; Protein folding; Proteinase inhibitors; Proteins; RNA, Messenger - metabolism; Schistocerca gregaria; Serine; Serine proteinase; Silk; Small heat shock proteins; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0121296

Small heat shock proteins (sHSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi response to microorganisms, a sHsp gene termed as Ap-sHSP21.4, was identified. This gene encoded a 21.4 kDa protein which shares the conserved structure of insect sHsps and belongs to sHSP21.4 family. Ap-sHSP21.4 was highly expressed in fat body and up-regulated in midgut and fat body of A. pernyi challenged with Escherichia coli, Beauveria bassiana and nuclear polyhedrosis virus (NPV), which was determined by quantitative real-time PCR. Meanwhile, knock down of Ap-sHSP21.4 with dsRNA result in the decrease at the expression levels of several immune response-related genes (defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor). Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge. All together, Ap-sHSP21.4 was involved in the immunity of A. pernyi against microorganism and possibly mediated by eicosanoids pathway. These results will shed light in the understanding of the pathogen-host interaction in A. pernyi.