Exposure to Leishmania braziliensis triggers neutrophil activation and apoptosis

• Published in: PLoS neglected tropical diseases 2015-03, Vol.9 (3), p.e0003601-e0003601
• Main Authors: Falcão, Sarah A C, Weinkopff, Tiffany, Hurrell, Benjamin P, Celes, Fabiana S, Curvelo, Rebecca P, Prates, Deboraci B, Barral, Aldina, Borges, Valeria M, Tacchini-Cottier, Fabienne, de Oliveira, Camila I
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; CD18 Antigens - analysis; Experiments; Female; Flow cytometry; Health aspects; Histograms; Infections; L-Selectin - analysis; Leishmania; Leishmania - immunology; Leishmania braziliensis; Leishmania braziliensis - immunology; Leukocyte Elastase - biosynthesis; Macrophages - immunology; Macrophages - parasitology; Medical research; Mice; Mice, Inbred BALB C; Neutrophil Activation; Neutrophil Infiltration - immunology; Neutrophils; Neutrophils - immunology; Parasites; Parasitic diseases; Superoxides - metabolism; Tumor Necrosis Factor-alpha - biosynthesis
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0003601

Neutrophils are the first line of defense against invading pathogens and are rapidly recruited to the sites of Leishmania inoculation. During Leishmania braziliensis infection, depletion of inflammatory cells significantly increases the parasite load whereas co-inoculation of neutrophils plus L. braziliensis had an opposite effect. Moreover, the co-culture of infected macrophages and neutrophils also induced parasite killing leading us to ask how neutrophils alone respond to an L. braziliensis exposure. Herein we focused on understanding the interaction between neutrophils and L. braziliensis, exploring cell activation and apoptotic fate. Inoculation of serum-opsonized L. braziliensis promastigotes in mice induced neutrophil accumulation in vivo, peaking at 24 h. In vitro, exposure of thyoglycollate-elicited inflammatory or bone marrow neutrophils to L. braziliensis modulated the expression of surface molecules such as CD18 and CD62L, and induced the oxidative burst. Using mCherry-expressing L. braziliensis, we determined that such effects were mainly observed in infected and not in bystander cells. Neutrophil activation following contact with L. braziliensis was also confirmed by the release of TNF-α and neutrophil elastase. Lastly, neutrophils infected with L. braziliensis but not with L. major displayed markers of early apoptosis. We show that L. braziliensis induces neutrophil recruitment in vivo and that neutrophils exposed to the parasite in vitro respond through activation and release of inflammatory mediators. This outcome may impact on parasite elimination, particularly at the early stages of infection.