CpG-ODN Class C Mediated Immunostimulation in Rabbit Model of Trypanosoma evansi Infection

CpG oligodeoxynucleotides (CpG-ODN) stimulate immune cells from a wide spectrum of mammalian species. Class C CpG-ODN is relatively stable and has the combined immune effects of both A and B classes of CpG-ODN. Trypanosoma evansi produces the state of immuno-suppression in the infected hosts. The cu...

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Bibliographic Details
Published in:PloS one 2015-06, Vol.10 (6), p.e0127437-e0127437
Main Authors: Kumar, Parveen, Kumar, Rakesh, Manuja, Balvinder Kumar, Singha, Harisankar, Sharma, Anshu, Virmani, Nitin, Yadav, Suresh Chandra, Manuja, Anju
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - pharmacology
Animal sciences
Animals
Antigens
Bubalus bubalis
Chemotherapy
CpG islands
Deoxyribonucleic acid
Disease Models, Animal
DNA
Follicles
Genetic aspects
Immune response
Immune response (humoral)
Immune system
Immunostimulation
Infections
Infiltration
Laboratory animals
Liver
Lymphocytes B
Mammals
Nanotechnology
Oligodeoxyribonucleotides - pharmacology
Oligonucleotides
Parasites
Pathology
Physiological aspects
Rabbits
Side effects
Spleen
Trypanosoma
Trypanosoma - immunology
Trypanosoma evansi
Trypanosomiasis - drug therapy
Trypanosomiasis - immunology
Trypanosomiasis - pathology
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Summary:CpG oligodeoxynucleotides (CpG-ODN) stimulate immune cells from a wide spectrum of mammalian species. Class C CpG-ODN is relatively stable and has the combined immune effects of both A and B classes of CpG-ODN. Trypanosoma evansi produces the state of immuno-suppression in the infected hosts. The current chemotherapeutic agents against this parasite are limited in number and usually associated with severe side effects. The present work aimed to determine the immunostimulatory effects of CpG-ODN class C in T. evansi infected rabbits. Rabbits inoculated with CpG C and challenged with T. evansi resulted in delayed onset of clinical signs with reduced severity in comparison to that of T. evansi infected rabbits. The treatment also enhanced humoral immune responses. Histopathological findings in liver and spleen revealed enhancement of mononuclear cell infiltration and secondary B cell follicles. These results demonstrate that CpG-ODN class C, has immunostimulatory properties in rabbit model of trypanosomosis. The use of booster doses or sustained delivery of CpG-ODN will further elucidate the prolonged CpG-ODN generated immune responses.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0127437