Influence of Nano-HA Coated Bone Collagen to Acrylic (Polymethylmethacrylate) Bone Cement on Mechanical Properties and Bioactivity

• Published in: PloS one 2015-06, Vol.10 (6), p.e0129018-e0129018
• Main Authors: Li, Tao, Weng, Xisheng, Bian, Yanyan, Zhou, Lei, Cui, Fuzhai, Qiu, Zhiye
• Format: Article
• Language: English
• Subjects: Animals; Biocompatibility; Biocompatible Materials; Biological activity; Biomarkers - metabolism; Biomechanical Phenomena; Biomechanics; Biomedical materials; Biosafety; Bone cements; Bone Cements - chemistry; Bone Cements - pharmacology; Bone implants; Bone surgery; Bone-implant interfaces; Bones; Carbon dioxide; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Cement; Chemical properties; Coagulation; Collagen; Collagen - chemistry; Collagen - pharmacology; Collagens; Composite materials; Compression; Compressive Strength; Connective tissue; Crosslinking; Cytotoxicity; Durapatite - chemistry; Durapatite - pharmacology; Experiments; Femur; Femur - drug effects; Femur - injuries; Femur - metabolism; Fibroblasts; Gene Expression; Humans; Hydroxyapatite; Implantation; Integrin-Binding Sialoprotein - genetics; Integrin-Binding Sialoprotein - metabolism; Joint surgery; Kidneys; Laboratory animals; Materials science; Materials Testing; Mechanical properties; Medical research; Medical schools; Mice; Modulus of elasticity; Nanostructures; Orthopedics; Osteocalcin - genetics; Osteocalcin - metabolism; Osteogenesis; Osteogenesis - drug effects; Osteonectin - genetics; Osteonectin - metabolism; Physiological aspects; Polymers; Polymethyl methacrylate; Polymethyl Methacrylate - chemistry; Polymethyl Methacrylate - pharmacology; Polymethylmethacrylate; Prostheses; Rabbits; Sodium; Solidification; Studies; Surgical implants; Tissues; Toxicity
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0129018

This research investigated the mechanical properties and bioactivity of polymethylmethacrylate (PMMA) bone cement after addition of the nano-hydroxyapatite(HA) coated bone collagen (mineralized collagen, MC). The MC in different proportions were added to the PMMA bone cement to detect the compressive strength, compression modulus, coagulation properties and biosafety. The MC-PMMA was embedded into rabbits and co-cultured with MG 63 cells to exam bone tissue compatibility and gene expression of osteogenesis. 15.0%(wt) impregnated MC-PMMA significantly lowered compressive modulus while little affected compressive strength and solidification. MC-PMMA bone cement was biologically safe and indicated excellent bone tissue compatibility. The bone-cement interface crosslinking was significantly higher in MC-PMMA than control after 6 months implantation in the femur of rabbits. The genes of osteogenesis exhibited significantly higher expression level in MC-PMMA. MC-PMMA presented perfect mechanical properties, good biosafety and excellent biocompatibility with bone tissues, which has profoundly clinical values.