Comparison of Aggregometry with Flow Cytometry for the Assessment of Agonists´-Induced Platelet Reactivity in Patients on Dual Antiplatelet Therapy

Data on the agreement between aggregometry and platelet activation by flow cytometry regarding the measurement of on-treatment platelet reactivity to arachidonic acid (AA) and adenosine diphosphate (ADP) are scarce. We therefore sought to compare three platelet aggregation tests with flow cytometry...

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Bibliographic Details
Published in:PloS one 2015-06, Vol.10 (6), p.e0129666-e0129666
Main Authors: Gremmel, Thomas, Koppensteiner, Renate, Panzer, Simon
Format: Article
Language:English
Subjects:
Adenosine
Adenosine diphosphate
Adenosine Diphosphate - pharmacology
Aged
Agglomeration
Angioplasty
Anticoagulants
Arachidonic acid
Arachidonic Acid - pharmacology
Aspirin
Assaying
ATPases
Blood platelets
Blood tests
Cardiovascular disease
Clopidogrel
Comparative analysis
Coronary vessels
Correlation
Cytometry
Drug Therapy, Combination
Flow
Flow cytometry
Flow Cytometry - methods
Glycoproteins
Humans
Implantation
Implants
In vivo methods and tests
Internal medicine
Light
Light effects
Light transmission
Middle Aged
P-selectin
Patients
Platelet aggregation
Platelet Aggregation - drug effects
Platelet Aggregation Inhibitors - pharmacology
Platelet Function Tests - methods
Reactivity
Sodium
Stents
Surgical implants
Therapy
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Summary:Data on the agreement between aggregometry and platelet activation by flow cytometry regarding the measurement of on-treatment platelet reactivity to arachidonic acid (AA) and adenosine diphosphate (ADP) are scarce. We therefore sought to compare three platelet aggregation tests with flow cytometry for the assessment of the response to antiplatelet therapy. Platelet aggregation in response to AA and ADP was determined by light transmission aggregometry (LTA), the VerifyNow assays, and multiple electrode aggregometry (MEA) in 316 patients receiving aspirin and clopidogrel therapy after angioplasty with stent implantation. AA- and ADP-induced P-selectin expression and activated glycoprotein (GP) IIb/IIIa were determined by flow cytometry. LTA, the VerifyNow P2Y12 assay and MEA in response to ADP correlated significantly (all p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0129666