LASP1, a Newly Identified Melanocytic Protein with a Possible Role in Melanin Release, but Not in Melanoma Progression

The LIM and SH3 protein 1 (LASP1) is a focal adhesion protein. Its expression is increased in many malignant tumors. However, little is known about the physiological role of the protein. In the present study, we investigated the expression and function of LASP1 in normal skin, melanocytic nevi and m...

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Bibliographic Details
Published in:PloS one 2015-06, Vol.10 (6), p.e0129219-e0129219
Main Authors: Vaman V S, Anjana, Poppe, Heiko, Houben, Roland, Grunewald, Thomas G P, Goebeler, Matthias, Butt, Elke
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing - genetics
Adaptor Proteins, Signal Transducing - metabolism
Biochemistry
Breast cancer
Cell Adhesion
Cell Movement
Cell Proliferation
Cells, Cultured
Cytoskeletal Proteins - genetics
Cytoskeletal Proteins - metabolism
Dermatology
Dynamin
Dynamins - metabolism
Gene Knockdown Techniques
Humans
Kinases
LIM Domain Proteins - genetics
LIM Domain Proteins - metabolism
Medical prognosis
Melanin
Melanins - metabolism
Melanocytes
Melanocytes - metabolism
Melanoma
Melanoma - genetics
Melanoma - metabolism
Melanosomes
Metastasis
mRNA
Nevus, Pigmented - genetics
Nevus, Pigmented - metabolism
Phase transitions
Phosphorylation
Physiological aspects
Physiology
Protein binding
Proteins
Skin
Skin diseases
Tips
Tumors
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Summary:The LIM and SH3 protein 1 (LASP1) is a focal adhesion protein. Its expression is increased in many malignant tumors. However, little is known about the physiological role of the protein. In the present study, we investigated the expression and function of LASP1 in normal skin, melanocytic nevi and malignant melanoma. In normal skin, a distinct LASP1 expression is visible only in the basal epidermal layer while in nevi LASP1 protein is detected in all melanocytes. Melanoma exhibit no increase in LASP1 mRNA compared to normal skin. In melanocytes, the protein is bound to dynamin and mainly localized at late melanosomes along the edges and at the tips of the cell. Knockdown of LASP1 results in increased melanin concentration in the cells. Collectively, we identified LASP1 as a hitherto unknown protein in melanocytes and as novel partner of dynamin in the physiological process of membrane constriction and melanosome vesicle release.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0129219