Prevalence of the Prefoldin Subunit 5 Gene Deletion in Canine Mammary Tumors

A somatic deletion at the proximal end of canine chromosome 27 (CFA27) was recently reported in 50% of malignant mammary tumors. This region harbours the tumor suppressor gene prefoldin subunit 5 (PFDN5) and the deletion correlated with a higher Ki-67 score. PFDN5 has been described to repress c-MYC...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-07, Vol.10 (7), p.e0131280
Main Authors: Hennecke, Silvia, Beck, Julia, Bornemann-Kolatzki, Kirsten, Neumann, Stephan, Murua Escobar, Hugo, Nolte, Ingo, Hammer, Susanne Conradine, Hewicker-Trautwein, Marion, Junginger, Johannes, Kaup, Franz-Josef, Brenig, Bertram, Schütz, Ekkehard
Format: Article
Language:English
Subjects:
Animals
Benign
Breast cancer
c-Myc protein
Cancer
Cancer genetics
Carcinogenesis
Carcinogens
Carcinoma
Cell growth
Chromosome deletion
Chromosomes
Copy number
Deoxyribonucleic acid
DNA
Dogs
Female
Gene Deletion
Genes
Genomes
Hematology
Immunohistochemistry
Leukocytes
Mammary Neoplasms, Animal - genetics
Mastectomy
Medical research
Molecular Chaperones - genetics
Myc protein
Oncology
Palliative care
Pathology
Prevalence studies (Epidemiology)
Protein Subunits - genetics
Rodents
Thyroid gland
Tissues
Tumor suppressor genes
Tumors
Veterinary medicine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c692t-fe61dadd221a02fc76d1785cb22137ba3f2a63122e3f3a6fc11889508cbe99673
cites cdi_FETCH-LOGICAL-c692t-fe61dadd221a02fc76d1785cb22137ba3f2a63122e3f3a6fc11889508cbe99673
container_end_page
container_issue 7
container_start_page e0131280
container_title PloS one
container_volume 10
creator Hennecke, Silvia
Beck, Julia
Bornemann-Kolatzki, Kirsten
Neumann, Stephan
Murua Escobar, Hugo
Nolte, Ingo
Hammer, Susanne Conradine
Hewicker-Trautwein, Marion
Junginger, Johannes
Kaup, Franz-Josef
Brenig, Bertram
Schütz, Ekkehard
description A somatic deletion at the proximal end of canine chromosome 27 (CFA27) was recently reported in 50% of malignant mammary tumors. This region harbours the tumor suppressor gene prefoldin subunit 5 (PFDN5) and the deletion correlated with a higher Ki-67 score. PFDN5 has been described to repress c-MYC and is, therefore, a candidate tumor-suppressor and cancer-driver gene in canine mammary cancer. Aim of this study was to confirm the recurrent deletion in a larger number of tumors. Droplet digital PCR for PFDN5 was performed in DNA from 102 malignant, 40 benign mammary tumors/dysplasias, 11 non-neoplastic mammary tissues and each corresponding genomic DNA from leukocytes. The copy number of PFDN5 was normalized to a reference amplicon on canine chromosome 32 (CFA32). Z-scores were calculated, based on Gaussian distributed normalized PFDN5 copy numbers of the leukocyte DNA. Z-scores ≤ -3.0 in tissue were considered as being indicative of the PFDN5 deletion and called as such. The Ki-67 proliferation index was assessed in a subset of 79 tissue samples by immunohistochemistry. The deletion was confirmed in 24% of all malignant tumors, detected in only 7.5% of the benign tumors and was not present in any normal mammary tissue sample. The subgroup of solid carcinomas (n = 9) showed the highest frequency of the deletion (67%) and those malignomas without microscopical high fraction of benign tissue (n = 71) had a 32% frequency (p
doi_str_mv 10.1371/journal.pone.0131280
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1692759580</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A420144594</galeid><doaj_id>oai_doaj_org_article_723fec76334a4b898f01526500b75f03</doaj_id><sourcerecordid>A420144594</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-fe61dadd221a02fc76d1785cb22137ba3f2a63122e3f3a6fc11889508cbe99673</originalsourceid><addsrcrecordid>eNqNkl-L1DAUxYso7jr6DUQLguDDjPnTpOmLsIy6DoysuKuvIU1vZjK0ydiki357M053mYKC5KHh3N89vb09WfYcowWmJX6780PvVLvYewcLhCkmAj3IznFFyZwTRB-e3M-yJyHsEGJUcP44OyMcU1JRfp6tv_Rwq1pwGnJv8riFPCnGt411-fVQD87GnOWX4CB_Dy1E612eSkvlbJI-q65T_a_8Zuh8H55mj4xqAzwbn7Ps28cPN8tP8_XV5Wp5sZ5rXpE4N8Bxo5qGEKwQMbrkDS4F03USaFkraoji6XsIUEMVNxpjISqGhK6hqnhJZ9nLo---9UGOiwgSJ_eSVUygRKyOROPVTu57e5hSemXlH8H3G6n6aHULsiTUQJqB0kIVtaiEQZgRzhCqS2YQTV7vxrcNdQeNBhd71U5MpxVnt3Ljb2VRiKqgh3FfjQa9_zFAiP8YeaQ26XdI64xPZrqzQcuLgiBcFCy5zbLFX6h0GuisTlEwNumThjeThsRE-Bk3aghBrq6__j979X3Kvj5ht6DauA2-HQ4BCVOwOIK69yGkbN1vDiN5SPLdNuQhyXJMcmp7cbr1-6a76NLfNH7rvQ</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1692759580</pqid></control><display><type>article</type><title>Prevalence of the Prefoldin Subunit 5 Gene Deletion in Canine Mammary Tumors</title><source>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</source><source>PubMed Central(OpenAccess)</source><creator>Hennecke, Silvia ; Beck, Julia ; Bornemann-Kolatzki, Kirsten ; Neumann, Stephan ; Murua Escobar, Hugo ; Nolte, Ingo ; Hammer, Susanne Conradine ; Hewicker-Trautwein, Marion ; Junginger, Johannes ; Kaup, Franz-Josef ; Brenig, Bertram ; Schütz, Ekkehard</creator><contributor>Liu, Xuefeng</contributor><creatorcontrib>Hennecke, Silvia ; Beck, Julia ; Bornemann-Kolatzki, Kirsten ; Neumann, Stephan ; Murua Escobar, Hugo ; Nolte, Ingo ; Hammer, Susanne Conradine ; Hewicker-Trautwein, Marion ; Junginger, Johannes ; Kaup, Franz-Josef ; Brenig, Bertram ; Schütz, Ekkehard ; Liu, Xuefeng</creatorcontrib><description>A somatic deletion at the proximal end of canine chromosome 27 (CFA27) was recently reported in 50% of malignant mammary tumors. This region harbours the tumor suppressor gene prefoldin subunit 5 (PFDN5) and the deletion correlated with a higher Ki-67 score. PFDN5 has been described to repress c-MYC and is, therefore, a candidate tumor-suppressor and cancer-driver gene in canine mammary cancer. Aim of this study was to confirm the recurrent deletion in a larger number of tumors. Droplet digital PCR for PFDN5 was performed in DNA from 102 malignant, 40 benign mammary tumors/dysplasias, 11 non-neoplastic mammary tissues and each corresponding genomic DNA from leukocytes. The copy number of PFDN5 was normalized to a reference amplicon on canine chromosome 32 (CFA32). Z-scores were calculated, based on Gaussian distributed normalized PFDN5 copy numbers of the leukocyte DNA. Z-scores ≤ -3.0 in tissue were considered as being indicative of the PFDN5 deletion and called as such. The Ki-67 proliferation index was assessed in a subset of 79 tissue samples by immunohistochemistry. The deletion was confirmed in 24% of all malignant tumors, detected in only 7.5% of the benign tumors and was not present in any normal mammary tissue sample. The subgroup of solid carcinomas (n = 9) showed the highest frequency of the deletion (67%) and those malignomas without microscopical high fraction of benign tissue (n = 71) had a 32% frequency (p&lt;0.01 vs. benign samples). The Ki-67 score was found to be significantly higher (p&lt;0.05) in the PFDN5-deleted group compared to malignant tumors without the deletion. A somatic deletion of the PFDN5 gene is recurrently present in canine mammary cancer, supporting a potential role in carcinogenesis. The association of this deletion with higher Ki-67 indicates an increased proliferation rate and thus a link to tumor aggressiveness can be hypothesized. The confirmation of earlier results warrants further studies on PFDN5 as cancer-driver gene.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0131280</identifier><identifier>PMID: 26132936</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Benign ; Breast cancer ; c-Myc protein ; Cancer ; Cancer genetics ; Carcinogenesis ; Carcinogens ; Carcinoma ; Cell growth ; Chromosome deletion ; Chromosomes ; Copy number ; Deoxyribonucleic acid ; DNA ; Dogs ; Female ; Gene Deletion ; Genes ; Genomes ; Hematology ; Immunohistochemistry ; Leukocytes ; Mammary Neoplasms, Animal - genetics ; Mastectomy ; Medical research ; Molecular Chaperones - genetics ; Myc protein ; Oncology ; Palliative care ; Pathology ; Prevalence studies (Epidemiology) ; Protein Subunits - genetics ; Rodents ; Thyroid gland ; Tissues ; Tumor suppressor genes ; Tumors ; Veterinary medicine</subject><ispartof>PloS one, 2015-07, Vol.10 (7), p.e0131280</ispartof><rights>COPYRIGHT 2015 Public Library of Science</rights><rights>2015 Hennecke et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Hennecke et al 2015 Hennecke et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-fe61dadd221a02fc76d1785cb22137ba3f2a63122e3f3a6fc11889508cbe99673</citedby><cites>FETCH-LOGICAL-c692t-fe61dadd221a02fc76d1785cb22137ba3f2a63122e3f3a6fc11889508cbe99673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1692759580/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1692759580?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26132936$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Liu, Xuefeng</contributor><creatorcontrib>Hennecke, Silvia</creatorcontrib><creatorcontrib>Beck, Julia</creatorcontrib><creatorcontrib>Bornemann-Kolatzki, Kirsten</creatorcontrib><creatorcontrib>Neumann, Stephan</creatorcontrib><creatorcontrib>Murua Escobar, Hugo</creatorcontrib><creatorcontrib>Nolte, Ingo</creatorcontrib><creatorcontrib>Hammer, Susanne Conradine</creatorcontrib><creatorcontrib>Hewicker-Trautwein, Marion</creatorcontrib><creatorcontrib>Junginger, Johannes</creatorcontrib><creatorcontrib>Kaup, Franz-Josef</creatorcontrib><creatorcontrib>Brenig, Bertram</creatorcontrib><creatorcontrib>Schütz, Ekkehard</creatorcontrib><title>Prevalence of the Prefoldin Subunit 5 Gene Deletion in Canine Mammary Tumors</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>A somatic deletion at the proximal end of canine chromosome 27 (CFA27) was recently reported in 50% of malignant mammary tumors. This region harbours the tumor suppressor gene prefoldin subunit 5 (PFDN5) and the deletion correlated with a higher Ki-67 score. PFDN5 has been described to repress c-MYC and is, therefore, a candidate tumor-suppressor and cancer-driver gene in canine mammary cancer. Aim of this study was to confirm the recurrent deletion in a larger number of tumors. Droplet digital PCR for PFDN5 was performed in DNA from 102 malignant, 40 benign mammary tumors/dysplasias, 11 non-neoplastic mammary tissues and each corresponding genomic DNA from leukocytes. The copy number of PFDN5 was normalized to a reference amplicon on canine chromosome 32 (CFA32). Z-scores were calculated, based on Gaussian distributed normalized PFDN5 copy numbers of the leukocyte DNA. Z-scores ≤ -3.0 in tissue were considered as being indicative of the PFDN5 deletion and called as such. The Ki-67 proliferation index was assessed in a subset of 79 tissue samples by immunohistochemistry. The deletion was confirmed in 24% of all malignant tumors, detected in only 7.5% of the benign tumors and was not present in any normal mammary tissue sample. The subgroup of solid carcinomas (n = 9) showed the highest frequency of the deletion (67%) and those malignomas without microscopical high fraction of benign tissue (n = 71) had a 32% frequency (p&lt;0.01 vs. benign samples). The Ki-67 score was found to be significantly higher (p&lt;0.05) in the PFDN5-deleted group compared to malignant tumors without the deletion. A somatic deletion of the PFDN5 gene is recurrently present in canine mammary cancer, supporting a potential role in carcinogenesis. The association of this deletion with higher Ki-67 indicates an increased proliferation rate and thus a link to tumor aggressiveness can be hypothesized. The confirmation of earlier results warrants further studies on PFDN5 as cancer-driver gene.</description><subject>Animals</subject><subject>Benign</subject><subject>Breast cancer</subject><subject>c-Myc protein</subject><subject>Cancer</subject><subject>Cancer genetics</subject><subject>Carcinogenesis</subject><subject>Carcinogens</subject><subject>Carcinoma</subject><subject>Cell growth</subject><subject>Chromosome deletion</subject><subject>Chromosomes</subject><subject>Copy number</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Dogs</subject><subject>Female</subject><subject>Gene Deletion</subject><subject>Genes</subject><subject>Genomes</subject><subject>Hematology</subject><subject>Immunohistochemistry</subject><subject>Leukocytes</subject><subject>Mammary Neoplasms, Animal - genetics</subject><subject>Mastectomy</subject><subject>Medical research</subject><subject>Molecular Chaperones - genetics</subject><subject>Myc protein</subject><subject>Oncology</subject><subject>Palliative care</subject><subject>Pathology</subject><subject>Prevalence studies (Epidemiology)</subject><subject>Protein Subunits - genetics</subject><subject>Rodents</subject><subject>Thyroid gland</subject><subject>Tissues</subject><subject>Tumor suppressor genes</subject><subject>Tumors</subject><subject>Veterinary medicine</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl-L1DAUxYso7jr6DUQLguDDjPnTpOmLsIy6DoysuKuvIU1vZjK0ydiki357M053mYKC5KHh3N89vb09WfYcowWmJX6780PvVLvYewcLhCkmAj3IznFFyZwTRB-e3M-yJyHsEGJUcP44OyMcU1JRfp6tv_Rwq1pwGnJv8riFPCnGt411-fVQD87GnOWX4CB_Dy1E612eSkvlbJI-q65T_a_8Zuh8H55mj4xqAzwbn7Ps28cPN8tP8_XV5Wp5sZ5rXpE4N8Bxo5qGEKwQMbrkDS4F03USaFkraoji6XsIUEMVNxpjISqGhK6hqnhJZ9nLo---9UGOiwgSJ_eSVUygRKyOROPVTu57e5hSemXlH8H3G6n6aHULsiTUQJqB0kIVtaiEQZgRzhCqS2YQTV7vxrcNdQeNBhd71U5MpxVnt3Ljb2VRiKqgh3FfjQa9_zFAiP8YeaQ26XdI64xPZrqzQcuLgiBcFCy5zbLFX6h0GuisTlEwNumThjeThsRE-Bk3aghBrq6__j979X3Kvj5ht6DauA2-HQ4BCVOwOIK69yGkbN1vDiN5SPLdNuQhyXJMcmp7cbr1-6a76NLfNH7rvQ</recordid><startdate>20150701</startdate><enddate>20150701</enddate><creator>Hennecke, Silvia</creator><creator>Beck, Julia</creator><creator>Bornemann-Kolatzki, Kirsten</creator><creator>Neumann, Stephan</creator><creator>Murua Escobar, Hugo</creator><creator>Nolte, Ingo</creator><creator>Hammer, Susanne Conradine</creator><creator>Hewicker-Trautwein, Marion</creator><creator>Junginger, Johannes</creator><creator>Kaup, Franz-Josef</creator><creator>Brenig, Bertram</creator><creator>Schütz, Ekkehard</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150701</creationdate><title>Prevalence of the Prefoldin Subunit 5 Gene Deletion in Canine Mammary Tumors</title><author>Hennecke, Silvia ; Beck, Julia ; Bornemann-Kolatzki, Kirsten ; Neumann, Stephan ; Murua Escobar, Hugo ; Nolte, Ingo ; Hammer, Susanne Conradine ; Hewicker-Trautwein, Marion ; Junginger, Johannes ; Kaup, Franz-Josef ; Brenig, Bertram ; Schütz, Ekkehard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-fe61dadd221a02fc76d1785cb22137ba3f2a63122e3f3a6fc11889508cbe99673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Benign</topic><topic>Breast cancer</topic><topic>c-Myc protein</topic><topic>Cancer</topic><topic>Cancer genetics</topic><topic>Carcinogenesis</topic><topic>Carcinogens</topic><topic>Carcinoma</topic><topic>Cell growth</topic><topic>Chromosome deletion</topic><topic>Chromosomes</topic><topic>Copy number</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Dogs</topic><topic>Female</topic><topic>Gene Deletion</topic><topic>Genes</topic><topic>Genomes</topic><topic>Hematology</topic><topic>Immunohistochemistry</topic><topic>Leukocytes</topic><topic>Mammary Neoplasms, Animal - genetics</topic><topic>Mastectomy</topic><topic>Medical research</topic><topic>Molecular Chaperones - genetics</topic><topic>Myc protein</topic><topic>Oncology</topic><topic>Palliative care</topic><topic>Pathology</topic><topic>Prevalence studies (Epidemiology)</topic><topic>Protein Subunits - genetics</topic><topic>Rodents</topic><topic>Thyroid gland</topic><topic>Tissues</topic><topic>Tumor suppressor genes</topic><topic>Tumors</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hennecke, Silvia</creatorcontrib><creatorcontrib>Beck, Julia</creatorcontrib><creatorcontrib>Bornemann-Kolatzki, Kirsten</creatorcontrib><creatorcontrib>Neumann, Stephan</creatorcontrib><creatorcontrib>Murua Escobar, Hugo</creatorcontrib><creatorcontrib>Nolte, Ingo</creatorcontrib><creatorcontrib>Hammer, Susanne Conradine</creatorcontrib><creatorcontrib>Hewicker-Trautwein, Marion</creatorcontrib><creatorcontrib>Junginger, Johannes</creatorcontrib><creatorcontrib>Kaup, Franz-Josef</creatorcontrib><creatorcontrib>Brenig, Bertram</creatorcontrib><creatorcontrib>Schütz, Ekkehard</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Science in Context</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing and Allied Health Journals</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>ProQuest Agriculture &amp; Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>ProQuest Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hennecke, Silvia</au><au>Beck, Julia</au><au>Bornemann-Kolatzki, Kirsten</au><au>Neumann, Stephan</au><au>Murua Escobar, Hugo</au><au>Nolte, Ingo</au><au>Hammer, Susanne Conradine</au><au>Hewicker-Trautwein, Marion</au><au>Junginger, Johannes</au><au>Kaup, Franz-Josef</au><au>Brenig, Bertram</au><au>Schütz, Ekkehard</au><au>Liu, Xuefeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence of the Prefoldin Subunit 5 Gene Deletion in Canine Mammary Tumors</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-07-01</date><risdate>2015</risdate><volume>10</volume><issue>7</issue><spage>e0131280</spage><pages>e0131280-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>A somatic deletion at the proximal end of canine chromosome 27 (CFA27) was recently reported in 50% of malignant mammary tumors. This region harbours the tumor suppressor gene prefoldin subunit 5 (PFDN5) and the deletion correlated with a higher Ki-67 score. PFDN5 has been described to repress c-MYC and is, therefore, a candidate tumor-suppressor and cancer-driver gene in canine mammary cancer. Aim of this study was to confirm the recurrent deletion in a larger number of tumors. Droplet digital PCR for PFDN5 was performed in DNA from 102 malignant, 40 benign mammary tumors/dysplasias, 11 non-neoplastic mammary tissues and each corresponding genomic DNA from leukocytes. The copy number of PFDN5 was normalized to a reference amplicon on canine chromosome 32 (CFA32). Z-scores were calculated, based on Gaussian distributed normalized PFDN5 copy numbers of the leukocyte DNA. Z-scores ≤ -3.0 in tissue were considered as being indicative of the PFDN5 deletion and called as such. The Ki-67 proliferation index was assessed in a subset of 79 tissue samples by immunohistochemistry. The deletion was confirmed in 24% of all malignant tumors, detected in only 7.5% of the benign tumors and was not present in any normal mammary tissue sample. The subgroup of solid carcinomas (n = 9) showed the highest frequency of the deletion (67%) and those malignomas without microscopical high fraction of benign tissue (n = 71) had a 32% frequency (p&lt;0.01 vs. benign samples). The Ki-67 score was found to be significantly higher (p&lt;0.05) in the PFDN5-deleted group compared to malignant tumors without the deletion. A somatic deletion of the PFDN5 gene is recurrently present in canine mammary cancer, supporting a potential role in carcinogenesis. The association of this deletion with higher Ki-67 indicates an increased proliferation rate and thus a link to tumor aggressiveness can be hypothesized. The confirmation of earlier results warrants further studies on PFDN5 as cancer-driver gene.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26132936</pmid><doi>10.1371/journal.pone.0131280</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2015-07, Vol.10 (7), p.e0131280
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1692759580
source Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central(OpenAccess)
subjects Animals
Benign
Breast cancer
c-Myc protein
Cancer
Cancer genetics
Carcinogenesis
Carcinogens
Carcinoma
Cell growth
Chromosome deletion
Chromosomes
Copy number
Deoxyribonucleic acid
DNA
Dogs
Female
Gene Deletion
Genes
Genomes
Hematology
Immunohistochemistry
Leukocytes
Mammary Neoplasms, Animal - genetics
Mastectomy
Medical research
Molecular Chaperones - genetics
Myc protein
Oncology
Palliative care
Pathology
Prevalence studies (Epidemiology)
Protein Subunits - genetics
Rodents
Thyroid gland
Tissues
Tumor suppressor genes
Tumors
Veterinary medicine
title Prevalence of the Prefoldin Subunit 5 Gene Deletion in Canine Mammary Tumors
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T20%3A47%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Prevalence%20of%20the%20Prefoldin%20Subunit%205%20Gene%20Deletion%20in%20Canine%20Mammary%20Tumors&rft.jtitle=PloS%20one&rft.au=Hennecke,%20Silvia&rft.date=2015-07-01&rft.volume=10&rft.issue=7&rft.spage=e0131280&rft.pages=e0131280-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0131280&rft_dat=%3Cgale_plos_%3EA420144594%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c692t-fe61dadd221a02fc76d1785cb22137ba3f2a63122e3f3a6fc11889508cbe99673%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1692759580&rft_id=info:pmid/26132936&rft_galeid=A420144594&rfr_iscdi=true