MUC1-Targeted Cancer Cell Photothermal Ablation Using Bioinspired Gold Nanorods

Recent studies have highlighted the overexpression of mucin 1 (MUC1) in various epithelial carcinomas and its role in tumorigenesis. These mucins present a novel targeting opportunity for nanoparticle-mediated photothermal cancer treatments due to their unique antenna-like extracellular extension. I...

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Bibliographic Details
Published in:PloS one 2015-07, Vol.10 (7), p.e0128756
Main Authors: Zelasko-Leon, Daria C, Fuentes, Christina M, Messersmith, Phillip B
Format: Article
Language:English
Subjects:
Ablation
Adhesives
Albumin
Antibodies
Bioengineering
Biomedical engineering
Biomimetics
Biotechnology
Breast cancer
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Breast Neoplasms - therapy
Cancer
Cancer therapies
Catechol
Cell Line, Tumor
Chemistry
Chemotherapy
Cytotoxicity
Engineering
Gold
Gold - chemistry
Growth factors
Humans
I.R. radiation
Immunoglobulins
Indoles - chemistry
Infrared lasers
Kinases
Mucin
Mucin-1 - metabolism
Mucins
Nanoparticles
Nanorods
Nanotubes
Phototherapy
Polymers - chemistry
Proteins
Serum Albumin, Bovine - chemistry
Squamous cell carcinoma
Toxicity
Tumorigenesis
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Summary:Recent studies have highlighted the overexpression of mucin 1 (MUC1) in various epithelial carcinomas and its role in tumorigenesis. These mucins present a novel targeting opportunity for nanoparticle-mediated photothermal cancer treatments due to their unique antenna-like extracellular extension. In this study, MUC1 antibodies and albumin were immobilized onto the surface of gold nanorods using a "primer" of polydopamine (PD), a molecular mimic of catechol- and amine-rich mussel adhesive proteins. PD forms an adhesive platform for the deposition of albumin and MUC1 antibodies, achieving a surface that is stable, bioinert and biofunctional. Two-photon luminescence confocal and darkfield scattering imaging revealed targeting of MUC1-BSA-PD-NRs to MUC1+ MCF-7 breast cancer and SCC-15 squamous cell carcinoma cells lines. Treated cells were exposed to a laser encompassing the near-infrared AuNR longitudinal surface plasmon and assessed for photothermal ablation. MUC1-BSA-PD-NRs substantially decreased cell viability in photoirradiated MCF-7 cell lines vs. MUC1- MDA-MB-231 breast cancer cells (p < 0.005). Agents exhibited no cytotoxicity in the absence of photothermal treatment. The facile nature of the coating method, combined with targeting and photoablation efficacy, are attractive features of these candidate cancer nanotherapeutics.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0128756