Whole Transcriptome of the Venom Gland from Urodacus yaschenkoi Scorpion

• Published in: PloS one 2015-05, Vol.10 (5), p.e0127883-e0127883
• Main Authors: Luna-Ramírez, Karen, Quintero-Hernández, Verónica, Juárez-González, Víctor Rivelino, Possani, Lourival D
• Format: Article
• Language: English
• Subjects: Allergens; Animals; Antimicrobial peptides; Arthropod Proteins - biosynthesis; Arthropod Proteins - genetics; Bioactive compounds; Biochemistry; Biological activity; Buthidae; Communications systems; Enzymes; Exocrine Glands - metabolism; Gene expression; Gene Expression Regulation - physiology; Genes; Health aspects; Health problems; Hymenoptera; Ion channels; Mass spectrometry; Peptides; Pharmacology; Protease inhibitors; Proteasomes; Proteinase inhibitors; Proteins; Proteomes; Ribosomal proteins; Scientific imaging; Scorpion Venoms - biosynthesis; Scorpion Venoms - genetics; Scorpions; Scorpions - genetics; Scorpions - metabolism; Sodium; Studies; Toxins; Transcriptome - physiology; Urodacus yaschenkoi; Venom; Venom gland; Venom toxins; Venoms
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0127883

Australian scorpion venoms have been poorly studied, probably because they do not pose an evident threat to humans. In addition, the continent has other medically important venomous animals capable of causing serious health problems. Urodacus yaschenkoi belongs to the most widely distributed family of Australian scorpions (Urodacidae) and it is found all over the continent, making it a useful model system for studying venom composition and evolution. This communication reports the whole set of mRNA transcripts produced by the venom gland. U. yaschenkoi venom is as complex as its overseas counterparts. These transcripts certainly code for several components similar to known scorpion venom components, such as: alpha-KTxs, beta-KTxs, calcins, protease inhibitors, antimicrobial peptides, sodium-channel toxins, toxin-like peptides, allergens, La1-like, hyaluronidases, ribosomal proteins, proteasome components and proteins related to cellular processes. A comparison with the venom gland transcriptome of Centruroides noxius (Buthidae) showed that these two scorpions have similar components related to biological processes, although important differences occur among the venom toxins. In contrast, a comparison with sequences reported for Urodacus manicatus revealed that these two Urodacidae species possess the same subfamily of scorpion toxins. A comparison with sequences of an U. yaschenkoi cDNA library previously reported by our group showed that both techniques are reliable for the description of the venom components, but the whole transcriptome generated with Next Generation Sequencing platform provides sequences of all transcripts expressed. Several of which were identified in the proteome, but many more transcripts were identified including uncommon transcripts. The information reported here constitutes a reference for non-Buthidae scorpion venoms, providing a comprehensive view of genes that are involved in venom production. Further, this work identifies new putative bioactive compounds that could be used to seed research into new pharmacological compounds and increase our understanding of the function of different ion channels.