Interspecific Phylogenic Relationships within Genus Melilotus Based on Nuclear and Chloroplast DNA

Melilotus comprises 19 species, while the phylogenetic relationships between species remain unclear. In the present work, three chloroplast genes, rbcL, matK, trnL-F, and one nuclear region, ITS (internal transcribed spacer) belonging to 48 populations of 18 species of Melilotus were sequenced and p...

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Bibliographic Details
Published in:PloS one 2015-07, Vol.10 (7), p.e0132596-e0132596
Main Authors: Di, Hongyan, Duan, Zhen, Luo, Kai, Zhang, Daiyu, Wu, Fan, Zhang, Jiyu, Liu, Wenxian, Wang, Yanrong
Format: Article
Language:English
Subjects:
Analysis
Asteraceae
Bayesian analysis
Bioinformatics
Cell Nucleus - genetics
Chloroplast DNA
Chloroplasts
Color
Data processing
Deoxyribonucleic acid
DNA
DNA, Chloroplast - genetics
Ecosystems
Genes
Genes, Plant
Genetic testing
Grasslands
Interspecific
Interspecific relationships
Laboratories
Legumes
Melilotus
Melilotus - classification
Melilotus - genetics
Morphology
Phylogenetics
Phylogeny
Plant reproduction
Science
Spacer
Species
Studies
Taxonomy
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Summary:Melilotus comprises 19 species, while the phylogenetic relationships between species remain unclear. In the present work, three chloroplast genes, rbcL, matK, trnL-F, and one nuclear region, ITS (internal transcribed spacer) belonging to 48 populations of 18 species of Melilotus were sequenced and phylogenetic trees were constructed to study their interspecific relationships. Based on the phylogenetic tree generated in this study using rbcL analysis, the Melilotus genus is clearly monophyletic in the legume family. Both Bayesian and maximum-parsimony approaches were used to analyze the data. The nrDNA ITS provided more informative characteristics (9.8%) than cpDNA (3.0%). Melilotus contains two closely related groups, clade I and clade II. M. spicatus, M. indicus and M. segetalis have a close relationship. M. infestus, M. siculus and M. sulcatus are closely related. The comparing between molecular phylogeny and flower color classification in Melilotus showed that the flower color is not much informative for phylogenetics of this genus.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0132596