Therapeutic Effects of Bupleurum Polysaccharides in Streptozotocin Induced Diabetic Mice

• Published in: PloS one 2015-07, Vol.10 (7), p.e0133212-e0133212
• Main Authors: Pan, Lingyu, Weng, Hongbo, Li, Hong, Liu, Zhenzhen, Xu, Yanyan, Zhou, Chunjiao, Lu, Xiaoxiao, Su, Xiaoyu, Zhang, Yunyi, Chen, Daofeng
• Format: Article
• Language: English
• Subjects: Animal experimentation; Animals; Anti-inflammatory agents; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - therapeutic use; Antioxidants - chemistry; Antioxidants - therapeutic use; Apoptosis; Blood; Blood glucose; Blood Glucose - metabolism; Body weight; Body Weight - drug effects; Bupleurum; Bupleurum - chemistry; Chinese medicine; Cholesterol; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; Diabetes Mellitus, Experimental - pathology; Drug dosages; Enzymes; Glucose; Glycogen; Health aspects; Hepatocytes; Herbal medicine; Hyperglycemia; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - therapeutic use; Inflammation; Insulin; Insulin - blood; Insulin - metabolism; Insulin resistance; Laboratory animals; Liver; Male; Medical research; Metabolism; Mice; Mice, Inbred C57BL; Oral administration; Oxidative stress; Pancreas; Pancreas - drug effects; Pancreas - metabolism; Pancreas - pathology; Pancreatic beta cells; Pharmacology; Pharmacy; Physiology; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - therapeutic use; Rodents; Saccharides; Streptozocin; Toxicology; Urine
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0133212

Diabetes mellitus is related to low-grade chronic inflammation and oxidative stress. Bupleurum Polysaccharides (BPs), isolated from Bupleurum smithii var. parvifolium has anti-inflammatory and anti-oxidative properties. However, little is known about its therapeutic effects on diabetes. In this experiment, the effects of BPs on alleviation of diabetes and the underlying mechanisms were investigated. Diabetic mice model was established via successive intraperitoneal injections of streptozotocin (100 mg/kg body weight) for two days. Mice with blood glucose levels higher than 16.8mmol/L were selected for experiments. The diabetic mice were orally administered with BPs (30 and 60 mg/kg) once a day for 35 days. BPs not only significantly decreased levels of blood glucose, but also increased those of serum insulin and liver glycogen in diabetic mice compared to model mice. Additionally, BPs adminstration improved the insulin expression and suppressed the apoptosis in pancreas of the diabetic mice. Histopathological observations further demonstrated that BPs protected the pancreas and liver from oxidative and inflammatory damages. These results suggest that BPs protect pancreatic β cells and liver hepatocytes and ameliorate diabetes, which is associated with its anti-oxidative and anti-inflammatory properties.