Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination

Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription. Multiple biological processes have been suggested to be tightly related to the dynamics of H2B monoubiquitination. However, a comprehensive understand...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-07, Vol.10 (7), p.e0133444-e0133444
Main Authors: Wu, You, Chen, Ping, Jing, Yuanya, Wang, Chen, Men, Yu-Long, Zhan, Wang, Wang, Qiang, Gan, Zhixue, Huang, Jin, Xie, Kun, Mi, Jiangsheng, Yu, Chenghua, Yu, Xiuqing, Chen, Pei-Chao, Chang, Jian-Feng, Cai, Fengfeng, Chen, Su
Format: Article
Language:English
Subjects:
Animals
Bioinformatics
Biological activity
Biological effects
Breast cancer
Cell cycle
Cell Differentiation - genetics
Chromatin
Chromatin - metabolism
Data processing
Deoxyribonucleic acid
DNA
DNA Damage - genetics
DNA methylation
DNA microarrays
DNA Repair - genetics
Education
Gene Expression Profiling
Gene Expression Regulation
Gene Knockdown Techniques
Genes
Genomes
HEK293 Cells
HeLa Cells
Histone H2B
Histones - genetics
Histones - metabolism
Humans
Life sciences
Mammals
Medicine
Mice
Mouse Embryonic Stem Cells - cytology
Mutant Proteins - metabolism
Mutation - genetics
Oligonucleotide Array Sequence Analysis
Stem cells
Surgery
Transcription
Tumorigenesis
Ubiquitin - metabolism
Ubiquitination
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c526t-7fea30f495c12f72abea9235ae811e72645a6f6004a83eb12a569f54aa5544623
cites cdi_FETCH-LOGICAL-c526t-7fea30f495c12f72abea9235ae811e72645a6f6004a83eb12a569f54aa5544623
container_end_page e0133444
container_issue 7
container_start_page e0133444
container_title PloS one
container_volume 10
creator Wu, You
Chen, Ping
Jing, Yuanya
Wang, Chen
Men, Yu-Long
Zhan, Wang
Wang, Qiang
Gan, Zhixue
Huang, Jin
Xie, Kun
Mi, Jiangsheng
Yu, Chenghua
Yu, Xiuqing
Chen, Pei-Chao
Chang, Jian-Feng
Cai, Fengfeng
Chen, Su
description Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription. Multiple biological processes have been suggested to be tightly related to the dynamics of H2B monoubiquitination. However, a comprehensive understanding of biological roles of H2B monoubiquitination is still poorly understood. In the present study, we developed an efficient tool to disrupt endogenous H2B monoubiquitination levels by using an H2BK120R mutant construct expressed in human cells. Genome-wide microarray analysis of these cells revealed a potential global view of biological functions of H2B monoubiquitination. Bioinformatics analysis of our data demonstrated that while H2B monoubiquitination expectedly affected a number of previously reported biological pathways, we also uncovered the influence of this histone modification on many novel biological processes. Therefore, our work provided valuable information for understanding the role of H2B monoubiquitination and indicated potential directions for its further studies.
doi_str_mv 10.1371/journal.pone.0133444
format article
fullrecord <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1696687427</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_b7f3878ecefd4033a4f15086d9455a88</doaj_id><sourcerecordid>3744631051</sourcerecordid><originalsourceid>FETCH-LOGICAL-c526t-7fea30f495c12f72abea9235ae811e72645a6f6004a83eb12a569f54aa5544623</originalsourceid><addsrcrecordid>eNptkl1rFDEUhoMotlb_geiAN97smu9kboS2WLfQoojeCeFMNlmzZCfbZKaw_95sd1pa8SqH5D3P-ciL0FuC54Qp8mmdxtxDnG9T7-aYMMY5f4aOScvoTFLMnj-Kj9CrUtYYC6alfImOqCRKMamO0e_rYHOCnGHXnFbcroTS_HC3DmJpvqfB9UOA2JyFFNMq2BpejL0dQupLk3yzCGWo9ZsFPWuuU5_GLtyMYQg97CWv0QtfOe7NdJ6gXxdffp4vZlffvl6en17NrKBymCnvgGHPW2EJ9YpC56ClTIDThDhFJRcgvcSYg2auIxSEbL3gAEJwLik7Qe8P3G1MxUyLKYbIVkqtOFVVcXlQLBOszTaHDeSdSRDM3UXKKwN5CDY60ynPtNLOOr_kmDHgngis5bLlQoDWlfV5qjZ2G7e0dUUZ4hPo05c-_DGrdGu4qDjBK-DjBMjpZnRlMJtQrIsRepfGu76VEoLxtko__CP9_3T8oKpfWUp2_qEZgs3eLPdZZm8WM5mlpr17PMhD0r072F-HF727</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1696687427</pqid></control><display><type>article</type><title>Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Wu, You ; Chen, Ping ; Jing, Yuanya ; Wang, Chen ; Men, Yu-Long ; Zhan, Wang ; Wang, Qiang ; Gan, Zhixue ; Huang, Jin ; Xie, Kun ; Mi, Jiangsheng ; Yu, Chenghua ; Yu, Xiuqing ; Chen, Pei-Chao ; Chang, Jian-Feng ; Cai, Fengfeng ; Chen, Su</creator><contributor>Mantovani, Roberto</contributor><creatorcontrib>Wu, You ; Chen, Ping ; Jing, Yuanya ; Wang, Chen ; Men, Yu-Long ; Zhan, Wang ; Wang, Qiang ; Gan, Zhixue ; Huang, Jin ; Xie, Kun ; Mi, Jiangsheng ; Yu, Chenghua ; Yu, Xiuqing ; Chen, Pei-Chao ; Chang, Jian-Feng ; Cai, Fengfeng ; Chen, Su ; Mantovani, Roberto</creatorcontrib><description>Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription. Multiple biological processes have been suggested to be tightly related to the dynamics of H2B monoubiquitination. However, a comprehensive understanding of biological roles of H2B monoubiquitination is still poorly understood. In the present study, we developed an efficient tool to disrupt endogenous H2B monoubiquitination levels by using an H2BK120R mutant construct expressed in human cells. Genome-wide microarray analysis of these cells revealed a potential global view of biological functions of H2B monoubiquitination. Bioinformatics analysis of our data demonstrated that while H2B monoubiquitination expectedly affected a number of previously reported biological pathways, we also uncovered the influence of this histone modification on many novel biological processes. Therefore, our work provided valuable information for understanding the role of H2B monoubiquitination and indicated potential directions for its further studies.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0133444</identifier><identifier>PMID: 26177367</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Bioinformatics ; Biological activity ; Biological effects ; Breast cancer ; Cell cycle ; Cell Differentiation - genetics ; Chromatin ; Chromatin - metabolism ; Data processing ; Deoxyribonucleic acid ; DNA ; DNA Damage - genetics ; DNA methylation ; DNA microarrays ; DNA Repair - genetics ; Education ; Gene Expression Profiling ; Gene Expression Regulation ; Gene Knockdown Techniques ; Genes ; Genomes ; HEK293 Cells ; HeLa Cells ; Histone H2B ; Histones - genetics ; Histones - metabolism ; Humans ; Life sciences ; Mammals ; Medicine ; Mice ; Mouse Embryonic Stem Cells - cytology ; Mutant Proteins - metabolism ; Mutation - genetics ; Oligonucleotide Array Sequence Analysis ; Stem cells ; Surgery ; Transcription ; Tumorigenesis ; Ubiquitin - metabolism ; Ubiquitination</subject><ispartof>PloS one, 2015-07, Vol.10 (7), p.e0133444-e0133444</ispartof><rights>2015 Wu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Wu et al 2015 Wu et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-7fea30f495c12f72abea9235ae811e72645a6f6004a83eb12a569f54aa5544623</citedby><cites>FETCH-LOGICAL-c526t-7fea30f495c12f72abea9235ae811e72645a6f6004a83eb12a569f54aa5544623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1696687427/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1696687427?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26177367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mantovani, Roberto</contributor><creatorcontrib>Wu, You</creatorcontrib><creatorcontrib>Chen, Ping</creatorcontrib><creatorcontrib>Jing, Yuanya</creatorcontrib><creatorcontrib>Wang, Chen</creatorcontrib><creatorcontrib>Men, Yu-Long</creatorcontrib><creatorcontrib>Zhan, Wang</creatorcontrib><creatorcontrib>Wang, Qiang</creatorcontrib><creatorcontrib>Gan, Zhixue</creatorcontrib><creatorcontrib>Huang, Jin</creatorcontrib><creatorcontrib>Xie, Kun</creatorcontrib><creatorcontrib>Mi, Jiangsheng</creatorcontrib><creatorcontrib>Yu, Chenghua</creatorcontrib><creatorcontrib>Yu, Xiuqing</creatorcontrib><creatorcontrib>Chen, Pei-Chao</creatorcontrib><creatorcontrib>Chang, Jian-Feng</creatorcontrib><creatorcontrib>Cai, Fengfeng</creatorcontrib><creatorcontrib>Chen, Su</creatorcontrib><title>Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription. Multiple biological processes have been suggested to be tightly related to the dynamics of H2B monoubiquitination. However, a comprehensive understanding of biological roles of H2B monoubiquitination is still poorly understood. In the present study, we developed an efficient tool to disrupt endogenous H2B monoubiquitination levels by using an H2BK120R mutant construct expressed in human cells. Genome-wide microarray analysis of these cells revealed a potential global view of biological functions of H2B monoubiquitination. Bioinformatics analysis of our data demonstrated that while H2B monoubiquitination expectedly affected a number of previously reported biological pathways, we also uncovered the influence of this histone modification on many novel biological processes. Therefore, our work provided valuable information for understanding the role of H2B monoubiquitination and indicated potential directions for its further studies.</description><subject>Animals</subject><subject>Bioinformatics</subject><subject>Biological activity</subject><subject>Biological effects</subject><subject>Breast cancer</subject><subject>Cell cycle</subject><subject>Cell Differentiation - genetics</subject><subject>Chromatin</subject><subject>Chromatin - metabolism</subject><subject>Data processing</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Damage - genetics</subject><subject>DNA methylation</subject><subject>DNA microarrays</subject><subject>DNA Repair - genetics</subject><subject>Education</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Gene Knockdown Techniques</subject><subject>Genes</subject><subject>Genomes</subject><subject>HEK293 Cells</subject><subject>HeLa Cells</subject><subject>Histone H2B</subject><subject>Histones - genetics</subject><subject>Histones - metabolism</subject><subject>Humans</subject><subject>Life sciences</subject><subject>Mammals</subject><subject>Medicine</subject><subject>Mice</subject><subject>Mouse Embryonic Stem Cells - cytology</subject><subject>Mutant Proteins - metabolism</subject><subject>Mutation - genetics</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Stem cells</subject><subject>Surgery</subject><subject>Transcription</subject><subject>Tumorigenesis</subject><subject>Ubiquitin - metabolism</subject><subject>Ubiquitination</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkl1rFDEUhoMotlb_geiAN97smu9kboS2WLfQoojeCeFMNlmzZCfbZKaw_95sd1pa8SqH5D3P-ciL0FuC54Qp8mmdxtxDnG9T7-aYMMY5f4aOScvoTFLMnj-Kj9CrUtYYC6alfImOqCRKMamO0e_rYHOCnGHXnFbcroTS_HC3DmJpvqfB9UOA2JyFFNMq2BpejL0dQupLk3yzCGWo9ZsFPWuuU5_GLtyMYQg97CWv0QtfOe7NdJ6gXxdffp4vZlffvl6en17NrKBymCnvgGHPW2EJ9YpC56ClTIDThDhFJRcgvcSYg2auIxSEbL3gAEJwLik7Qe8P3G1MxUyLKYbIVkqtOFVVcXlQLBOszTaHDeSdSRDM3UXKKwN5CDY60ynPtNLOOr_kmDHgngis5bLlQoDWlfV5qjZ2G7e0dUUZ4hPo05c-_DGrdGu4qDjBK-DjBMjpZnRlMJtQrIsRepfGu76VEoLxtko__CP9_3T8oKpfWUp2_qEZgs3eLPdZZm8WM5mlpr17PMhD0r072F-HF727</recordid><startdate>20150715</startdate><enddate>20150715</enddate><creator>Wu, You</creator><creator>Chen, Ping</creator><creator>Jing, Yuanya</creator><creator>Wang, Chen</creator><creator>Men, Yu-Long</creator><creator>Zhan, Wang</creator><creator>Wang, Qiang</creator><creator>Gan, Zhixue</creator><creator>Huang, Jin</creator><creator>Xie, Kun</creator><creator>Mi, Jiangsheng</creator><creator>Yu, Chenghua</creator><creator>Yu, Xiuqing</creator><creator>Chen, Pei-Chao</creator><creator>Chang, Jian-Feng</creator><creator>Cai, Fengfeng</creator><creator>Chen, Su</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20150715</creationdate><title>Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination</title><author>Wu, You ; Chen, Ping ; Jing, Yuanya ; Wang, Chen ; Men, Yu-Long ; Zhan, Wang ; Wang, Qiang ; Gan, Zhixue ; Huang, Jin ; Xie, Kun ; Mi, Jiangsheng ; Yu, Chenghua ; Yu, Xiuqing ; Chen, Pei-Chao ; Chang, Jian-Feng ; Cai, Fengfeng ; Chen, Su</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-7fea30f495c12f72abea9235ae811e72645a6f6004a83eb12a569f54aa5544623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Bioinformatics</topic><topic>Biological activity</topic><topic>Biological effects</topic><topic>Breast cancer</topic><topic>Cell cycle</topic><topic>Cell Differentiation - genetics</topic><topic>Chromatin</topic><topic>Chromatin - metabolism</topic><topic>Data processing</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Damage - genetics</topic><topic>DNA methylation</topic><topic>DNA microarrays</topic><topic>DNA Repair - genetics</topic><topic>Education</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Gene Knockdown Techniques</topic><topic>Genes</topic><topic>Genomes</topic><topic>HEK293 Cells</topic><topic>HeLa Cells</topic><topic>Histone H2B</topic><topic>Histones - genetics</topic><topic>Histones - metabolism</topic><topic>Humans</topic><topic>Life sciences</topic><topic>Mammals</topic><topic>Medicine</topic><topic>Mice</topic><topic>Mouse Embryonic Stem Cells - cytology</topic><topic>Mutant Proteins - metabolism</topic><topic>Mutation - genetics</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Stem cells</topic><topic>Surgery</topic><topic>Transcription</topic><topic>Tumorigenesis</topic><topic>Ubiquitin - metabolism</topic><topic>Ubiquitination</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, You</creatorcontrib><creatorcontrib>Chen, Ping</creatorcontrib><creatorcontrib>Jing, Yuanya</creatorcontrib><creatorcontrib>Wang, Chen</creatorcontrib><creatorcontrib>Men, Yu-Long</creatorcontrib><creatorcontrib>Zhan, Wang</creatorcontrib><creatorcontrib>Wang, Qiang</creatorcontrib><creatorcontrib>Gan, Zhixue</creatorcontrib><creatorcontrib>Huang, Jin</creatorcontrib><creatorcontrib>Xie, Kun</creatorcontrib><creatorcontrib>Mi, Jiangsheng</creatorcontrib><creatorcontrib>Yu, Chenghua</creatorcontrib><creatorcontrib>Yu, Xiuqing</creatorcontrib><creatorcontrib>Chen, Pei-Chao</creatorcontrib><creatorcontrib>Chang, Jian-Feng</creatorcontrib><creatorcontrib>Cai, Fengfeng</creatorcontrib><creatorcontrib>Chen, Su</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies &amp; Aerospace Collection</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Advanced Technologies &amp; Aerospace Database</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, You</au><au>Chen, Ping</au><au>Jing, Yuanya</au><au>Wang, Chen</au><au>Men, Yu-Long</au><au>Zhan, Wang</au><au>Wang, Qiang</au><au>Gan, Zhixue</au><au>Huang, Jin</au><au>Xie, Kun</au><au>Mi, Jiangsheng</au><au>Yu, Chenghua</au><au>Yu, Xiuqing</au><au>Chen, Pei-Chao</au><au>Chang, Jian-Feng</au><au>Cai, Fengfeng</au><au>Chen, Su</au><au>Mantovani, Roberto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2015-07-15</date><risdate>2015</risdate><volume>10</volume><issue>7</issue><spage>e0133444</spage><epage>e0133444</epage><pages>e0133444-e0133444</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription. Multiple biological processes have been suggested to be tightly related to the dynamics of H2B monoubiquitination. However, a comprehensive understanding of biological roles of H2B monoubiquitination is still poorly understood. In the present study, we developed an efficient tool to disrupt endogenous H2B monoubiquitination levels by using an H2BK120R mutant construct expressed in human cells. Genome-wide microarray analysis of these cells revealed a potential global view of biological functions of H2B monoubiquitination. Bioinformatics analysis of our data demonstrated that while H2B monoubiquitination expectedly affected a number of previously reported biological pathways, we also uncovered the influence of this histone modification on many novel biological processes. Therefore, our work provided valuable information for understanding the role of H2B monoubiquitination and indicated potential directions for its further studies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>26177367</pmid><doi>10.1371/journal.pone.0133444</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2015-07, Vol.10 (7), p.e0133444-e0133444
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1696687427
source Publicly Available Content Database; PubMed Central
subjects Animals
Bioinformatics
Biological activity
Biological effects
Breast cancer
Cell cycle
Cell Differentiation - genetics
Chromatin
Chromatin - metabolism
Data processing
Deoxyribonucleic acid
DNA
DNA Damage - genetics
DNA methylation
DNA microarrays
DNA Repair - genetics
Education
Gene Expression Profiling
Gene Expression Regulation
Gene Knockdown Techniques
Genes
Genomes
HEK293 Cells
HeLa Cells
Histone H2B
Histones - genetics
Histones - metabolism
Humans
Life sciences
Mammals
Medicine
Mice
Mouse Embryonic Stem Cells - cytology
Mutant Proteins - metabolism
Mutation - genetics
Oligonucleotide Array Sequence Analysis
Stem cells
Surgery
Transcription
Tumorigenesis
Ubiquitin - metabolism
Ubiquitination
title Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T18%3A02%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Microarray%20Analysis%20Reveals%20Potential%20Biological%20Functions%20of%20Histone%20H2B%20Monoubiquitination&rft.jtitle=PloS%20one&rft.au=Wu,%20You&rft.date=2015-07-15&rft.volume=10&rft.issue=7&rft.spage=e0133444&rft.epage=e0133444&rft.pages=e0133444-e0133444&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0133444&rft_dat=%3Cproquest_plos_%3E3744631051%3C/proquest_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c526t-7fea30f495c12f72abea9235ae811e72645a6f6004a83eb12a569f54aa5544623%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1696687427&rft_id=info:pmid/26177367&rfr_iscdi=true