Correlation of Real Time PCR Cycle Threshold Cut-Off with Bordetella pertussis Clinical Severity

Bordetella pertussis testing performed using real-time polymerase chain reaction (RT-PCR) is interpreted based on a cycle threshold (Ct) value. At Public Health Ontario Laboratories (PHOL), a Ct value

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Bibliographic Details
Published in:PloS one 2015-07, Vol.10 (7), p.e0133209-e0133209
Main Authors: Bolotin, Shelly, Deeks, Shelley L, Marchand-Austin, Alex, Rilkoff, Heather, Dang, Vica, Walton, Ryan, Hashim, Ahmed, Farrell, David, Crowcroft, Natasha S
Format: Article
Language:English
Subjects:
Adolescent
Adult
Age Distribution
Aged
Aged, 80 and over
Bordetella
Bordetella pertussis
Bordetella pertussis - genetics
Child
Child, Preschool
Disease control
Epidemiology
Health Information Systems
Hospitalization
Humans
Infant
Infant, Newborn
Information systems
Laboratories
Medical informatics
Medical personnel
Middle Aged
Odds Ratio
Ontario
Patients
Pertussis
Physicians
Polymerase chain reaction
Predictive Value of Tests
Public Health
Real time
Real-Time Polymerase Chain Reaction - methods
Severity of Illness Index
Vaccination
Vaccines
Whooping cough
Whooping Cough - microbiology
Whooping Cough - pathology
Young Adult
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Description
Summary:Bordetella pertussis testing performed using real-time polymerase chain reaction (RT-PCR) is interpreted based on a cycle threshold (Ct) value. At Public Health Ontario Laboratories (PHOL), a Ct value
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0133209