Immunohistochemistry and Fluorescence In Situ Hybridization Can Inform the Differential Diagnosis of Low-Grade Noninvasive Urothelial Carcinoma with an Inverted Growth Pattern and Inverted Urothelial Papilloma

Urothelial carcinoma (UC) comprises a heterogeneous group of epithelial neoplasms with diverse biological behaviors and variable clinical outcomes. Distinguishing UC histological subtypes has become increasingly important because prognoses and therapy can dramatically differ among subtypes. In clini...

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Published in:PloS one 2015-07, Vol.10 (7), p.e0133530-e0133530
Main Authors: Sun, Juan-Juan, Wu, Yong, Lu, Yong-Ming, Zhang, Hui-Zhi, Wang, Tao, Yang, Xiao-Qun, Sun, Meng-Hong, Wang, Chao-Fu
Format: Article
Language:English
Subjects:
Biomarkers, Tumor
Bladder cancer
Carcinoma
Carcinoma - diagnosis
Carcinoma - mortality
Cell cycle
Chromosomes
Consent
Cytogenetics
Cytokeratin
Diagnosis
Diagnosis, Differential
Differential diagnosis
Ethics
Fluorescence
Fluorescence in situ hybridization
Growth
Humans
Hybridization
Immunohistochemistry
In Situ Hybridization, Fluorescence
Kaplan-Meier Estimate
Labeling
Medical diagnosis
Medical research
Neoplasia
Neoplasm Grading
Neoplasms
Oncology
p53 Protein
Papilloma
Papilloma, Inverted - diagnosis
Pathology
Prognosis
Tumor proteins
Tumors
Urologic Neoplasms - diagnosis
Urologic Neoplasms - mortality
Urothelial carcinoma
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cited_by cdi_FETCH-LOGICAL-c692t-a24b359c178f2bda87e3dca1919d4a8f52903b5532afd992fc3648375332f4283
cites cdi_FETCH-LOGICAL-c692t-a24b359c178f2bda87e3dca1919d4a8f52903b5532afd992fc3648375332f4283
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container_issue 7
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container_title PloS one
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creator Sun, Juan-Juan
Wu, Yong
Lu, Yong-Ming
Zhang, Hui-Zhi
Wang, Tao
Yang, Xiao-Qun
Sun, Meng-Hong
Wang, Chao-Fu
description Urothelial carcinoma (UC) comprises a heterogeneous group of epithelial neoplasms with diverse biological behaviors and variable clinical outcomes. Distinguishing UC histological subtypes has become increasingly important because prognoses and therapy can dramatically differ among subtypes. In clinical work, overlapping morphological findings between low-grade noninvasive UC (LGNUC), which exhibits an inverted growth pattern, and inverted urothelial papilloma (IUP) can make subclassification difficult. We propose a combination of immunohistochemistry (IHC) and molecular cytogenetics for subtyping these clinical entities. In our study, tissue microarray immunohistochemical profiles of Ki-67, p53, cytokeratin 20 (CK20) and cyclinD1 were assessed. Molecular genetic alterations such as the gain of chromosomes 3, 7 or 17 or the homozygous loss of 9p21 were also assessed for their usefulness in differentiating these conditions. Based on our analysis, Ki-67 and CK20 may be useful for the differential diagnosis of these two tumor types. Fluorescence in situ hybridization (FISH) can also provide important data in cases in which the malignant nature of an inverted urothelial neoplasm is unclear. LGNUC with an inverted growth pattern that is negative for both Ki-67 and CK20 can be positively detected using FISH.
doi_str_mv 10.1371/journal.pone.0133530
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Distinguishing UC histological subtypes has become increasingly important because prognoses and therapy can dramatically differ among subtypes. In clinical work, overlapping morphological findings between low-grade noninvasive UC (LGNUC), which exhibits an inverted growth pattern, and inverted urothelial papilloma (IUP) can make subclassification difficult. We propose a combination of immunohistochemistry (IHC) and molecular cytogenetics for subtyping these clinical entities. In our study, tissue microarray immunohistochemical profiles of Ki-67, p53, cytokeratin 20 (CK20) and cyclinD1 were assessed. Molecular genetic alterations such as the gain of chromosomes 3, 7 or 17 or the homozygous loss of 9p21 were also assessed for their usefulness in differentiating these conditions. Based on our analysis, Ki-67 and CK20 may be useful for the differential diagnosis of these two tumor types. 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Distinguishing UC histological subtypes has become increasingly important because prognoses and therapy can dramatically differ among subtypes. In clinical work, overlapping morphological findings between low-grade noninvasive UC (LGNUC), which exhibits an inverted growth pattern, and inverted urothelial papilloma (IUP) can make subclassification difficult. We propose a combination of immunohistochemistry (IHC) and molecular cytogenetics for subtyping these clinical entities. In our study, tissue microarray immunohistochemical profiles of Ki-67, p53, cytokeratin 20 (CK20) and cyclinD1 were assessed. Molecular genetic alterations such as the gain of chromosomes 3, 7 or 17 or the homozygous loss of 9p21 were also assessed for their usefulness in differentiating these conditions. Based on our analysis, Ki-67 and CK20 may be useful for the differential diagnosis of these two tumor types. 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source Open Access: PubMed Central; ProQuest - Publicly Available Content Database
subjects Biomarkers, Tumor
Bladder cancer
Carcinoma
Carcinoma - diagnosis
Carcinoma - mortality
Cell cycle
Chromosomes
Consent
Cytogenetics
Cytokeratin
Diagnosis
Diagnosis, Differential
Differential diagnosis
Ethics
Fluorescence
Fluorescence in situ hybridization
Growth
Humans
Hybridization
Immunohistochemistry
In Situ Hybridization, Fluorescence
Kaplan-Meier Estimate
Labeling
Medical diagnosis
Medical research
Neoplasia
Neoplasm Grading
Neoplasms
Oncology
p53 Protein
Papilloma
Papilloma, Inverted - diagnosis
Pathology
Prognosis
Tumor proteins
Tumors
Urologic Neoplasms - diagnosis
Urologic Neoplasms - mortality
Urothelial carcinoma
title Immunohistochemistry and Fluorescence In Situ Hybridization Can Inform the Differential Diagnosis of Low-Grade Noninvasive Urothelial Carcinoma with an Inverted Growth Pattern and Inverted Urothelial Papilloma
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T07%3A53%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immunohistochemistry%20and%20Fluorescence%20In%20Situ%20Hybridization%20Can%20Inform%20the%20Differential%20Diagnosis%20of%20Low-Grade%20Noninvasive%20Urothelial%20Carcinoma%20with%20an%20Inverted%20Growth%20Pattern%20and%20Inverted%20Urothelial%20Papilloma&rft.jtitle=PloS%20one&rft.au=Sun,%20Juan-Juan&rft.date=2015-07-24&rft.volume=10&rft.issue=7&rft.spage=e0133530&rft.epage=e0133530&rft.pages=e0133530-e0133530&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0133530&rft_dat=%3Cgale_plos_%3EA422999855%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c692t-a24b359c178f2bda87e3dca1919d4a8f52903b5532afd992fc3648375332f4283%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1698611549&rft_id=info:pmid/26208279&rft_galeid=A422999855&rfr_iscdi=true