Reduced Expression of TET1, TET2, TET3 and TDG mRNAs Are Associated with Poor Prognosis of Patients with Early Breast Cancer

• Published in: PloS one 2015-07, Vol.10 (7), p.e0133896-e0133896
• Main Authors: Yang, Liu, Yu, San-Jian, Hong, Qi, Yang, Yu, Shao, Zhi-Ming
• Format: Article
• Language: English
• Subjects: Adult; Aged; Analysis; Anthracycline; Anthracyclines; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - mortality; Breast Neoplasms - pathology; Cancer; Cancer patients; Chemotherapy; Deoxyribonucleic acid; Dioxygenases - genetics; Dioxygenases - metabolism; Disease-Free Survival; DNA; DNA glycosylase; DNA Glycosylases - genetics; DNA Glycosylases - metabolism; DNA methylation; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Down-Regulation; Epidermal growth factor; Epigenetics; Female; Gene expression; Humans; Indicators; Leukemia; Medical diagnosis; Medical prognosis; Medical research; Melanoma; Messenger RNA; Metastasis; Middle Aged; Mixed Function Oxygenases; mRNA; Multivariate analysis; Mutation; Oncology; Oxidation; Patients; Polymerase chain reaction; Prognosis; Prostate cancer; Proteins; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; Regression analysis; RNA, Messenger - genetics; RNA, Messenger - metabolism; Skin cancer; Surgery; Survival; Survival Rate; Tissues; Translocation; Tumorigenesis; Tumors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0133896

The purpose of this study was to determine the prognostic role of ten eleven translocation (TET) family proteins and DNA glycosylase (TDG) in patients with early breast cancer (EBC). Expression of mRNAs encoding TET1-3 and TDG in 162 breast cancer tissues was quantified using real-time polymerase chain reaction analysis. The general characteristics of patients and clinicopathologic factors were collected. Estimation of patient survival was calculated using the Kaplan-Meier method, and independent prognostic indicators were analyzed using Cox regression analysis. The level of TET1 mRNA was significantly related to overall survival (OS) (P = 0.022). Multivariate analysis shows that the TNM stage was an independent predictor of disease-free survival (DFS) (HR = 1.761, 95% CI: 1.124-2.761, P = 0.014) and OS (HR = 2.135, 95% CI: 1.070-4.263, P = 0.032). Further, in patients with EBC who were treated with anthracyclines, Kaplan-Meier analysis indicates that the levels of TET3 and TDG mRNAs were related to DFS (P = 0.026 and 0.030, respectively), and multivariate analysis reveals that high levels of TET3 (HR = 1.944, 95% CI: 1.029-3.672, P = 0.040) and TDG (HR = 2.178, 95% CI: 1.140-4.163, P = 0.018) mRNAs were independent indicators of favorable DFS. Our study indicates that EBC patients with decreased expression of TET1 mRNA had worse OS and that the levels of TET3 and TDG mRNAs were independent prognostic factors for patients who received anthracycline chemotherapy.