Regulatory B Cell Function Is Suppressed by Smoking and Obesity in H. pylori-Infected Subjects and Is Correlated with Elevated Risk of Gastric Cancer

Helicobacter pylori infection occurs in more than half of the world's population and is the main cause for gastric cancer. A series of lifestyle and nutritional factors, such as tobacco smoking and obesity, have been found to elevate the risk for cancer development. In this study, we sought to...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-07, Vol.10 (7), p.e0134591-e0134591
Main Authors: Li, Guanggang, Wulan, Hasi, Song, Zongchang, Paik, Paul A, Tsao, Ming L, Goodman, Gary M, MacEachern, Paul T, Downey, Robert S, Jankowska, Anna J, Rabinowitz, Yaron M, Learch, Thomas B, Song, David Z, Yuan, Ji J, Zheng, Shihang, Zheng, Zhendong
Format: Article
Language:English
Subjects:
ADP-ribosyl Cyclase 1 - physiology
Adult
Antigens
B cells
B-Lymphocytes - physiology
Cancer
CD24 Antigen - physiology
CD38 antigen
Comparative analysis
Cytokines
Development and progression
Female
Flow Cytometry
Gastric cancer
Health aspects
Health risks
Helicobacter infections
Helicobacter Infections - complications
Helicobacter pylori
HIV
Hospitals
Human immunodeficiency virus
Humans
Immunology
Infection
Infections
Inflammation
Interleukin 10
Lymphocyte receptors
Lymphocyte Subsets - physiology
Lymphocytes
Lymphocytes B
Lymphocytes T
Male
Middle Aged
Obesity
Obesity - complications
Obesity - immunology
Oncology
Patients
Regulation
Risk Factors
Smoking
Smoking - adverse effects
Stimulation
Stomach cancer
Stomach Neoplasms - etiology
T cells
Tobacco
Tobacco smoking
Tumor necrosis factor-TNF
Tumorigenesis
Ulcers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Helicobacter pylori infection occurs in more than half of the world's population and is the main cause for gastric cancer. A series of lifestyle and nutritional factors, such as tobacco smoking and obesity, have been found to elevate the risk for cancer development. In this study, we sought to determine the immunological aspects during H. pylori infection and gastric cancer development. We found that B cells from H. pylori-infected patients presented altered composition and function compared to uninfected patients. IL-10-expressing CD24+CD38+ B cells were upregulated in H. pylori-infected patients, contained potent regulatory activity in inhibiting T cell pro-inflammatory cytokine secretion, and responded directly to H. pylori antigen stimulation. Interestingly, in H. pylori-infected smoking subjects and obese subjects, the number of IL-10+ B cells and CD24+CD38+ B cells were reduced compared to H. pylori-infected asymptomatic subjects. Regulatory functions mediated by CD24+CD38+ B cells were also impaired. In addition, gastric cancer positive patients had reduced IL-10-producing B cell frequencies after H. pylori-stimulation. Altogether, these data suggest that in H. pylori-infection, CD24+CD38+ B cell is upregulated and plays a role in suppressing pro-inflammatory responses, possibly through IL-10 production, a feature that was not observed in smoking and obese patients.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0134591