Effect of Low Doses (5-40 cGy) of Gamma-irradiation on Lifespan and Stress-related Genes Expression Profile in Drosophila melanogaster

• Published in: PloS one 2015-08, Vol.10 (8), p.e0133840
• Main Authors: Zhikrevetskaya, Svetlana, Peregudova, Darya, Danilov, Anton, Plyusnina, Ekaterina, Krasnov, George, Dmitriev, Alexey, Kudryavtseva, Anna, Shaposhnikov, Mikhail, Moskalev, Alexey
• Format: Article
• Language: English
• Subjects: Aerospace industry; Aerospace safety; Aging; Animals; Apoptosis; Cosmic rays; Deoxyribonucleic acid; Developmental stages; DNA; DNA repair; Drosophila; Drosophila melanogaster; Drosophila melanogaster - genetics; Drosophila melanogaster - metabolism; Drosophila melanogaster - radiation effects; Ecology; Environmental monitoring; Exposure; Female; Females; Gamma Rays; Gene expression; Genes; Genetic aspects; Genomes; Gerontology; Hormesis; Insects; Ionizing radiation; Irradiation; Laboratories; Life expectancy; Life span; Longevity - radiation effects; Male; Males; Mammals; Molecular biology; Oxidative stress; Physiological aspects; Physiology; Radiation; Radiation dosage; Radiation tolerance; Radiation, Ionizing; Real-Time Polymerase Chain Reaction; RNA - isolation & purification; RNA - metabolism; Science; Signal transduction; Transcriptome - radiation effects
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0133840

Studying of the effects of low doses of γ-irradiation is a crucial issue in different areas of interest, from environmental safety and industrial monitoring to aerospace and medicine. The goal of this work is to identify changes of lifespan and expression stress-sensitive genes in Drosophila melanogaster, exposed to low doses of γ-irradiation (5-40 cGy) on the imaginal stage of development. Although some changes in life extensity in males were identified (the effect of hormesis after the exposure to 5, 10 and 40 cGy) as well as in females (the effect of hormesis after the exposure to 5 and 40 cGy), they were not caused by the organism "physiological" changes. This means that the observed changes in life expectancy are not related to the changes of organism physiological functions after the exposure to low doses of ionizing radiation. The identified changes in gene expression are not dose-dependent, there is not any proportionality between dose and its impact on expression. These results reflect nonlinear effects of low dose radiation and sex-specific radio-resistance of the postmitotic cell state of Drosophila melanogaster imago.