Free Fatty Acid Effects on the Atrial Myocardium: Membrane Ionic Currents Are Remodeled by the Disruption of T-Tubular Architecture

Epicardial adiposity and plasma levels of free fatty acids (FFAs) are elevated in atrial fibrillation, heart failure and obesity, with potentially detrimental effects on myocardial function. As major components of epicardial fat, FFAs may be abnormally regulated, with a potential to detrimentally mo...

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Bibliographic Details
Published in:PloS one 2015-08, Vol.10 (8), p.e0133052-e0133052
Main Authors: O'Connell, Ryan P, Musa, Hassan, Gomez, Mario San Martin, Avula, Uma Mahesh, Herron, Todd J, Kalifa, Jerome, Anumonwo, Justus M B
Format: Article
Language:English
Subjects:
Action potential
Adipocytes
Adipose tissue
Animals
Atrial fibrillation
Biochemistry
Calcium
Calcium channels (L-type)
Cardiac arrhythmia
Cells, Cultured
Computer simulation
Electrophoresis, Polyacrylamide Gel
Electrophysiology
Experiments
Fatty acids
Fatty Acids, Nonesterified - pharmacology
Fibrillation
Glucose
Heart
Heart Atria - drug effects
Heart diseases
Heart failure
Immunoblotting
Incubation
Internal medicine
Ion channels
Ion Transport - drug effects
Laboratory animals
Male
Mathematical models
Mechanical properties
Medical research
Membrane capacitance
Membrane potential
Myocardium
Myocardium - cytology
Myocardium - metabolism
Myocytes
Numerical simulations
Obesity
Ostomy
Physiology
Plasma levels
Potassium
Properties (attributes)
Reduction
Saturated fatty acids
Sheep
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Summary:Epicardial adiposity and plasma levels of free fatty acids (FFAs) are elevated in atrial fibrillation, heart failure and obesity, with potentially detrimental effects on myocardial function. As major components of epicardial fat, FFAs may be abnormally regulated, with a potential to detrimentally modulate electro-mechanical function. The cellular mechanisms underlying such effects of FFAs are unknown. To determine the mechanisms underlying electrophysiological effects of palmitic (PA), stearic (SA) and oleic (OA) FFAs on sheep atrial myocytes. We used electrophysiological techniques, numerical simulations, biochemistry and optical imaging to examine the effects of acutely (≤ 15 min), short-term (4-6 hour) or 24-hour application of individual FFAs (10 μM) on isolated ovine left atrial myocytes (LAMs). Acute and short-term incubation in FFAs resulted in no differences in passive or active properties of isolated left atrial myocytes (LAMs). 24-hour application had differential effects depending on the FFA. PA did not affect cellular passive properties but shortened (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0133052