Genome Wide Binding Site Analysis Reveals Transcriptional Coactivation of Cytokinin-Responsive Genes by DELLA Proteins

The ability of plants to provide a plastic response to environmental cues relies on the connectivity between signaling pathways. DELLA proteins act as hubs that relay environmental information to the multiple transcriptional circuits that control growth and development through physical interaction w...

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Bibliographic Details
Published in:PLoS genetics 2015-07, Vol.11 (7), p.e1005337-e1005337
Main Authors: Marín-de la Rosa, Nora, Pfeiffer, Anne, Hill, Kristine, Locascio, Antonella, Bhalerao, Rishikesh P, Miskolczi, Pal, Grønlund, Anne L, Wanchoo-Kohli, Aakriti, Thomas, Stephen G, Bennett, Malcolm J, Lohmann, Jan U, Blázquez, Miguel A, Alabadí, David
Format: Article
Language:English
Subjects:
Arabidopsis - embryology
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Base Sequence
Binding Sites - genetics
Chromatin Immunoprecipitation
Councils
Cytokinins - metabolism
DNA, Plant - genetics
DNA-Binding Proteins - metabolism
Gene expression
Gene Expression Regulation, Plant
Genetics
Genetik
Genomes
Plant Development - physiology
Plant Roots - growth & development
Promoter Regions, Genetic - genetics
Proteins
Repressor Proteins - genetics
Repressor Proteins - metabolism
Scholarships & fellowships
Seeds
Sequence Analysis, DNA
Signal Transduction
Transcription factors
Transcription Factors - metabolism
Transcriptional Activation - genetics
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Summary:The ability of plants to provide a plastic response to environmental cues relies on the connectivity between signaling pathways. DELLA proteins act as hubs that relay environmental information to the multiple transcriptional circuits that control growth and development through physical interaction with transcription factors from different families. We have analyzed the presence of one DELLA protein at the Arabidopsis genome by chromatin immunoprecipitation coupled to large-scale sequencing and we find that it binds at the promoters of multiple genes. Enrichment analysis shows a strong preference for cis elements recognized by specific transcription factor families. In particular, we demonstrate that DELLA proteins are recruited by type-B ARABIDOPSIS RESPONSE REGULATORS (ARR) to the promoters of cytokinin-regulated genes, where they act as transcriptional co-activators. The biological relevance of this mechanism is underpinned by the necessity of simultaneous presence of DELLAs and ARRs to restrict root meristem growth and to promote photomorphogenesis.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1005337