Impaired Maternal Behavior in Usp46 Mutant Mice: A Model for Trans-Generational Transmission of Maternal Care

• Published in: PloS one 2015-08, Vol.10 (8), p.e0136016-e0136016
• Main Authors: Umemura, Shoya, Imai, Saki, Mimura, Ayumi, Fujiwara, Mari, Ebihara, Shizufumi
• Format: Article
• Language: English
• Subjects: Aggression; Analgesics; Animal behavior; Animal experimentation; Animals; Behavior; Behavior, Animal; Brain; Child abuse; Child abuse & neglect; Comparative analysis; DNA methylation; Endopeptidases - physiology; Epigenetics; Euthanasia; Experiments; Female; Genetic crosses; Genetics; Genotypes; House mouse; Human behavior; Inheritance Patterns - genetics; Laboratory animals; Latency; Maternal Behavior; Maternal Deprivation; Mathematical models; Mice; Mice, Inbred C57BL; Mothers; Mutation; Mutation - genetics; Neurosciences; Nurses; Nursing; Offspring; Parameters; Postpartum; Pregnancy; Psychobiology; Rodents; Science; Social Environment; Weaning
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0136016

Usp46 mutant mice (congenic strain on a B6 genetic background; MT mice) have a low weaning rate and display poor maternal behavior compared to C57BL/6J mice (B6 mice). Based on these observations, we examined how maternal behavior is shaped by cross-fostering and in-fostering MT and B6 mice. The experiments consisted of six groups: B6 mice fostered by their biological mother (B6-CO); MT mice fostered by their biological mother (MT-CO); B6 mice fostered by a different B6 mother (B6-IF); MT mice fostered by a different MT mother (MT-IF); B6 mice fostered by an MT mother (B6-CF); and MT mice fostered by a B6 mother (MT-CF). Maternal behavior was assessed using the pup-retrieval test in adult female offspring, and four parameters, time nursing pups in the nest, time sniffing or licking pups, rearing behavior, and latency to retrieve pups, were measured. Cross-fostering significantly reduced time spent nursing and sniffing/licking pup, and increased the number of instances of rearing in the B6-CF group, and improved three parameters of maternal behaviors (nursing, rearing and latency) in the MT-CF group. These results indicate that the level of maternal care is transmitted to their pups and proper maternal behaviors can be shaped if adequate postpartum maternal care is given, even in genetically vulnerable mice. However, the offspring's genotype may also influence the development of maternal behaviors in adulthood. Thus, MT mice may prove useful as a model for trans-generational transmission of maternal care, and these findings may provide insight into the mechanisms of maltreating behaviors in human child abuse.