Amniotic Fluid Derived Stem Cells with a Renal Progenitor Phenotype Inhibit Interstitial Fibrosis in Renal Ischemia and Reperfusion Injury in Rats

Mesenchymal stem cells derived from human amniotic fluid (hAFSCs) are a promising source for cellular therapy, especially for renal disorders, as a subpopulation is derived from the fetal urinary tract. The purpose of this study was to evaluate if hAFSCs with a renal progenitor phenotype demonstrate...

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Bibliographic Details
Published in:PloS one 2015-08, Vol.10 (8), p.e0136145-e0136145
Main Authors: Monteiro Carvalho Mori da Cunha, Marina Gabriela, Zia, Silvia, Oliveira Arcolino, Fanny, Carlon, Marianne Sylvia, Beckmann, Diego Vilibaldo, Pippi, Ney Luis, Luhers Graça, Dominguita, Levtchenko, Elena, Deprest, Jan, Toelen, Jaan
Format: Article
Language:English
Subjects:
Acute Kidney Injury - pathology
Acute Kidney Injury - therapy
Amniotic fluid
Amniotic Fluid - cytology
Animals
Bone marrow
Care and treatment
Causes of
Cell Differentiation
Cell Tracking
Cells, Cultured
Clear cell-type renal cell carcinoma
Creatinine
Female
Fetuses
Fibrosis
Genetic aspects
Humans
Infiltration
Inflammation
Injury prevention
Ischemia
Kidney - cytology
Kidney - pathology
Kidneys
Laboratories
Male
Mesenchymal stem cells
Mesenchyme
Nephrology
Pax2 protein
Pediatrics
Phenotypes
Physiological aspects
Physiology
Pregnancy
Progenitor cells
Proteins
Proteinuria
Rats
Rats, Wistar
Regeneration
Reperfusion
Reperfusion Injury - pathology
Reperfusion Injury - therapy
Rodents
Statistical analysis
Statistics
Stem Cell Transplantation
Stem cells
Stem Cells - cytology
Surgery
Urinary tract
Urine
Variance analysis
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Summary:Mesenchymal stem cells derived from human amniotic fluid (hAFSCs) are a promising source for cellular therapy, especially for renal disorders, as a subpopulation is derived from the fetal urinary tract. The purpose of this study was to evaluate if hAFSCs with a renal progenitor phenotype demonstrate a nephroprotective effect in acute ischemia reperfusion (I/R) model and prevent late stage fibrosis. A total of 45 male 12-wk-old Wistar rats were divided into three equal groups;: rats subjected to I/R injury and treated with Chang Medium, rats subjected to I/R injury and treated with hAFSCs and sham-operated animals. In the first part of this study, hAFSCs that highly expressed CD24, CD117, SIX2 and PAX2 were isolated and characterized. In the second part, renal I/R injury was induced in male rats and cellular treatment was performed 6 hours later via arterial injection. Functional and histological analyses were performed 24 hours, 48 hours and 2 months after treatment using serum creatinine, urine protein to creatinine ratio, inflammatory and regeneration markers and histomorphometric analysis of the kidney. Statistical analysis was performed by analysis of variance followed by the Tukey's test for multiple comparisons or by nonparametric Kruskal-Wallis followed by Dunn. Statistical significance level was defined as p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0136145