Novel Analysis Software for Detecting and Classifying Ca2+ Transient Abnormalities in Stem Cell-Derived Cardiomyocytes

Comprehensive functioning of Ca2+ cycling is crucial for excitation-contraction coupling of cardiomyocytes (CMs). Abnormal Ca2+ cycling is linked to arrhythmogenesis, which is associated with cardiac disorders and heart failure. Accordingly, we have generated spontaneously beating CMs from induced p...

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Published in:PloS one 2015-08, Vol.10 (8), p.e0135806-e0135806
Main Authors: Penttinen, Kirsi, Siirtola, Harri, Àvalos-Salguero, Jorge, Vainio, Tiina, Juhola, Martti, Aalto-Setälä, Katriina
Format: Article
Language:English
Subjects:
Abnormalities
Action Potentials - physiology
Calcium
Calcium - metabolism
Calcium ions
Calcium Signaling - physiology
Cardiac arrhythmia
Cardiomyocytes
Cardiomyopathy
Cells, Cultured
Classification
Computer programs
Contraction
Coronary artery disease
Cycles
Data analysis
Disease
Excitation Contraction Coupling
Hand tools
Heart
Heart diseases
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Mutation
Myocardial Contraction
Myocytes, Cardiac - cytology
Myocytes, Cardiac - metabolism
Pluripotency
Ryanodine Receptor Calcium Release Channel - metabolism
Software
Software development tools
Stem cells
Studies
Tachycardia
Tachycardia, Ventricular - physiopathology
Transient analysis
Usability
Ventricle
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cited_by cdi_FETCH-LOGICAL-c456t-a183aeb2b1095eff6314aa0bd43afa61a3dea3d9376b3a054bcaaa9b93458ebb3
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container_issue 8
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container_title PloS one
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creator Penttinen, Kirsi
Siirtola, Harri
Àvalos-Salguero, Jorge
Vainio, Tiina
Juhola, Martti
Aalto-Setälä, Katriina
description Comprehensive functioning of Ca2+ cycling is crucial for excitation-contraction coupling of cardiomyocytes (CMs). Abnormal Ca2+ cycling is linked to arrhythmogenesis, which is associated with cardiac disorders and heart failure. Accordingly, we have generated spontaneously beating CMs from induced pluripotent stem cells (iPSC) derived from patients with catecholaminergic polymorphic ventricular tachycardia (CPVT), which is an inherited and severe cardiac disease. Ca2+ cycling studies have revealed substantial abnormalities in these CMs. Ca2+ transient analysis performed manually lacks accepted analysis criteria, and has both low throughput and high variability. To overcome these issues, we have developed a software tool, AnomalyExplorer based on interactive visualization, to assist in the classification of Ca2+ transient patterns detected in CMs. Here, we demonstrate the usability and capability of the software, and we also compare the analysis efficiency to manual analysis. We show that AnomalyExplorer is suitable for detecting normal and abnormal Ca2+ transients; furthermore, this method provides more defined and consistent information regarding the Ca2+ abnormality patterns and cell line specific differences when compared to manual analysis. This tool will facilitate and speed up the analysis of CM Ca2+ transients, making it both more accurate and user-independent. AnomalyExplorer can be exploited in Ca2+ cycling analysis to study basic disease pathology and the effects of different drugs.
doi_str_mv 10.1371/journal.pone.0135806
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Abnormal Ca2+ cycling is linked to arrhythmogenesis, which is associated with cardiac disorders and heart failure. Accordingly, we have generated spontaneously beating CMs from induced pluripotent stem cells (iPSC) derived from patients with catecholaminergic polymorphic ventricular tachycardia (CPVT), which is an inherited and severe cardiac disease. Ca2+ cycling studies have revealed substantial abnormalities in these CMs. Ca2+ transient analysis performed manually lacks accepted analysis criteria, and has both low throughput and high variability. To overcome these issues, we have developed a software tool, AnomalyExplorer based on interactive visualization, to assist in the classification of Ca2+ transient patterns detected in CMs. Here, we demonstrate the usability and capability of the software, and we also compare the analysis efficiency to manual analysis. 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subjects Abnormalities
Action Potentials - physiology
Calcium
Calcium - metabolism
Calcium ions
Calcium Signaling - physiology
Cardiac arrhythmia
Cardiomyocytes
Cardiomyopathy
Cells, Cultured
Classification
Computer programs
Contraction
Coronary artery disease
Cycles
Data analysis
Disease
Excitation Contraction Coupling
Hand tools
Heart
Heart diseases
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Mutation
Myocardial Contraction
Myocytes, Cardiac - cytology
Myocytes, Cardiac - metabolism
Pluripotency
Ryanodine Receptor Calcium Release Channel - metabolism
Software
Software development tools
Stem cells
Studies
Tachycardia
Tachycardia, Ventricular - physiopathology
Transient analysis
Usability
Ventricle
title Novel Analysis Software for Detecting and Classifying Ca2+ Transient Abnormalities in Stem Cell-Derived Cardiomyocytes
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