Monitoring Spongospora subterranea Development in Potato Roots Reveals Distinct Infection Patterns and Enables Efficient Assessment of Disease Control Methods

• Published in: PloS one 2015-09, Vol.10 (9), p.e0137647
• Main Authors: Thangavel, Tamilarasan, Tegg, Robert S, Wilson, Calum R
• Format: Article
• Language: English
• Subjects: Control methods; Deoxyribonucleic acid; Disease control; Disease Resistance - genetics; Diseases and pests; DNA; Epidemics; Fungicides; Fungicides, Industrial - pharmacology; Genetic aspects; Host-parasite relationships; Infections; Inoculation; Laboratories; Mancozeb; Medical treatment; Monitoring; Multiplication; Pathogens; Pesticides; Physiological aspects; Plant diseases; Plant Diseases - microbiology; Plant Roots - drug effects; Plant Roots - microbiology; Plasmodiophorida - drug effects; Plasmodiophorida - pathogenicity; Potatoes; Roots; Seeds - drug effects; Seeds - microbiology; Soil Microbiology; Soil treatment; Solanum tuberosum - drug effects; Solanum tuberosum - microbiology; Spongospora subterranea; Streptomyces; Studies; Tomatoes; Tubers; Zoosporangia
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0137647

Spongospora subterranea is responsible for significant potato root and tuber disease globally. Study of this obligate (non-culturable) pathogen that infects below-ground plant parts is technically difficult. The capacity to measure the dynamics and patterns of root infections can greatly assist in determining the efficacy of control treatments on disease progression. This study used qPCR and histological analysis in time-course experiments to measure temporal patterns of pathogen multiplication and disease development in potato (and tomato) roots and tubers. Effects of delayed initiation of infection and fungicidal seed tuber and soil treatments were assessed. This study found roots at all plant developmental ages were susceptible to infection but that delaying infection significantly reduced pathogen content and resultant disease at final harvest. The pathogen was first detected in roots 15-20 days after inoculation (DAI) and the presence of zoosporangia noted 15-45 DAI. Following initial infection pathogen content in roots increased at a similar rate regardless of plant age at inoculation. All fungicide treatments (except soil-applied mancozeb which had a variable response) suppressed pathogen multiplication and root and tuber disease. In contrast to delayed inoculation, the fungicide treatments slowed disease progress (rate) rather than delaying onset of infection. Trials under suboptimal temperatures for disease expression provided valuable data on root infection rate, demonstrating the robustness of monitoring root infection. These results provide an early measure of the efficacy of control treatments and indicate two possible patterns of disease suppression by either delayed initiation of infection which then proceeds at a similar rate or diminished epidemic rate.