The Unc-5 Receptor Is Directly Regulated by Tinman in the Developing Drosophila Dorsal Vessel

During early heart morphogenesis cardiac cells migrate in two bilateral opposing rows, meet at the dorsal midline and fuse to form a hollow tube known as the primary heart field in vertebrates or dorsal vessel (DV) in Drosophila. Guidance receptors are thought to mediate this evolutionarily conserve...

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Published in:PloS one 2015-09, Vol.10 (9), p.e0137688-e0137688
Main Authors: Asadzadeh, Jamshid, Neligan, Niamh, Canabal-Alvear, Judith J, Daly, Amanda C, Kramer, Sunita Gupta, Labrador, Juan-Pablo
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding Sites
Cell migration
Cell receptors
Drosophila
Drosophila - embryology
Drosophila - genetics
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Enhancer Elements, Genetic
Evolutionary conservation
Gene Expression
Gene Expression Regulation, Developmental
Gene regulation
Genes
Genes, Reporter
Genetic aspects
Genetics
Heart
Heart - embryology
Heart diseases
Homology
Insects
Invertebrates
Laboratories
Microprocessors
Morphogenesis
Myoblasts, Cardiac - metabolism
Neurosciences
Nkx2.5 protein
Nucleotide Motifs
Organogenesis
Pathology
Physiological aspects
Protein Binding
Receptors
Repressor Proteins - metabolism
Sequence Alignment
Trans-Activators - metabolism
Transcription factors
Vertebrates
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cited_by cdi_FETCH-LOGICAL-c692t-3e0bc7ef43b39523671eded9675e32f646bdb04f8a88d5e68b664f19b30d103a3
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container_title PloS one
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Neligan, Niamh
Canabal-Alvear, Judith J
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Kramer, Sunita Gupta
Labrador, Juan-Pablo
description During early heart morphogenesis cardiac cells migrate in two bilateral opposing rows, meet at the dorsal midline and fuse to form a hollow tube known as the primary heart field in vertebrates or dorsal vessel (DV) in Drosophila. Guidance receptors are thought to mediate this evolutionarily conserved process. A core of transcription factors from the NK2, GATA and T-box families are also believed to orchestrate this process in both vertebrates and invertebrates. Nevertheless, whether they accomplish their function, at least in part, through direct or indirect transcriptional regulation of guidance receptors is currently unknown. In our work, we demonstrate how Tinman (Tin), the Drosophila homolog of the Nkx-2.5 transcription factor, regulates the Unc-5 receptor during DV tube morphogenesis. We use genetics, expression analysis with single cell mRNA resolution and enhancer-reporter assays in vitro or in vivo to demonstrate that Tin is required for Unc-5 receptor expression specifically in cardioblasts. We show that Tin can bind to evolutionary conserved sites within an Unc-5 DV enhancer and that these sites are required for Tin-dependent transactivation both in vitro and in vivo.
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subjects Animals
Base Sequence
Binding Sites
Cell migration
Cell receptors
Drosophila
Drosophila - embryology
Drosophila - genetics
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Enhancer Elements, Genetic
Evolutionary conservation
Gene Expression
Gene Expression Regulation, Developmental
Gene regulation
Genes
Genes, Reporter
Genetic aspects
Genetics
Heart
Heart - embryology
Heart diseases
Homology
Insects
Invertebrates
Laboratories
Microprocessors
Morphogenesis
Myoblasts, Cardiac - metabolism
Neurosciences
Nkx2.5 protein
Nucleotide Motifs
Organogenesis
Pathology
Physiological aspects
Protein Binding
Receptors
Repressor Proteins - metabolism
Sequence Alignment
Trans-Activators - metabolism
Transcription factors
Vertebrates
title The Unc-5 Receptor Is Directly Regulated by Tinman in the Developing Drosophila Dorsal Vessel
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