The Functional Study of the N-Terminal Region of Influenza B Virus Nucleoprotein

Influenza nucleoprotein (NP) is a major component of the ribonucleoprotein (vRNP) in influenza virus, which functions for the transcription and replication of viral genome. Compared to the nucleoprotein of influenza A (ANP), the N-terminal region of influenza B nucleoprotein (BNP) is much extended....

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Bibliographic Details
Published in:PloS one 2015-09, Vol.10 (9), p.e0137802-e0137802
Main Authors: Liu, Ming, Lam, Mandy Ka-Han, Zhang, Qinfen, Elderfield, Ruth, Barclay, Wendy S, Shaw, Pang-Chui
Format: Article
Language:English
Subjects:
Crystallography
Electron microscopy
Fluorescence
Fluorescence microscopy
Genomes
Genomics
Health aspects
Influenza
Influenza A
Influenza A virus - chemistry
Influenza A virus - metabolism
Influenza B
Influenza B virus - chemistry
Influenza B virus - metabolism
Life sciences
Light scattering
Microscopy
Oligomers
Plasmids
Protein Structure, Tertiary
Proteins
Ribonucleic acid
RNA
RNA, Viral - chemistry
RNA, Viral - metabolism
Transcription
Transcription (Genetics)
Viral Core Proteins - chemistry
Viral Core Proteins - metabolism
Virology
Viruses
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Summary:Influenza nucleoprotein (NP) is a major component of the ribonucleoprotein (vRNP) in influenza virus, which functions for the transcription and replication of viral genome. Compared to the nucleoprotein of influenza A (ANP), the N-terminal region of influenza B nucleoprotein (BNP) is much extended. By virus reconstitution, we found that the first 38 residues are essential for viral growth. We further illustrated the function of BNP by mini-genome reconstitution, fluorescence microscopy, electron microscopy, light scattering and gel shift. Results show that the N terminus is involved in the formation of both higher homo-oligomers of BNP and BNP-RNA complex.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0137802