Frequencies, Laboratory Features, and Granulocyte Activation in Chinese Patients with CALR-Mutated Myeloproliferative Neoplasms

• Published in: PloS one 2015-09, Vol.10 (9), p.e0138250-e0138250
• Main Authors: Guo, Haixiu, Chen, Xiuhua, Tian, Ruiyuan, Chang, Jianmei, Li, Jianlan, Tan, Yanhong, Xu, Zhifang, Ren, Fanggang, Zhao, Junxia, Pan, Jie, Zhang, Na, Wang, Xiaojuan, He, Jianxia, Yang, Wanfang, Wang, Hongwei
• Format: Article
• Language: English
• Subjects: Activation; Adolescent; Adult; Aged; Aged, 80 and over; Alkaline phosphatase; Alleles; Bands; Bone marrow; Calreticulin - genetics; Care and treatment; Cell activation; China - epidemiology; Cohort Studies; Development and progression; DNA sequencing; Female; Follow-Up Studies; Gene mutation; Genetic aspects; Granulocytes; Granulocytes - metabolism; Granulocytes - pathology; Hematology; Hemoglobin; Hospitals; Humans; Janus Kinase 2 - genetics; Kinases; Laboratories; Leukemia; Leukocytes; Leukocytes (granulocytic); Male; Medical records; Middle Aged; Mutants; Mutation; Mutation - genetics; Myelofibrosis; Myeloproliferative Disorders - epidemiology; Myeloproliferative Disorders - genetics; Myeloproliferative Disorders - pathology; Neoplasms; Patients; Phenotype; Phosphatase; Polycythemia; Polycythemia vera; Polymerase Chain Reaction; Prognosis; Receptors, Thrombopoietin - genetics; Thrombocythemia, Essential - epidemiology; Thrombocythemia, Essential - genetics; Thrombocythemia, Essential - pathology; Tumors; White blood cells; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0138250

Somatic mutations in the CALR gene have been recently identified as acquired alterations in myeloproliferative neoplasms (MPNs). In this study, we evaluated mutation frequencies, laboratory features, and granulocyte activation in Chinese patients with MPNs. A combination of qualitative allele-specific polymerase chain reaction and Sanger sequencing was used to detect three driver mutations (i.e., CALR, JAK2V617F, and MPL). CALR mutations were identified in 8.4% of cases with essential thrombocythemia (ET) and 5.3% of cases with primary myelofibrosis (PMF). Moreover, 25% of polycythemia vera, 29.5% of ET, and 48.1% of PMF were negative for all three mutations (JAK2V617F, MPL, and CALR). Compared with those patients with JAK2V617F mutation, CALR-mutated ET patients displayed unique hematological phenotypes, including higher platelet counts, and lower leukocyte counts and hemoglobin levels. Significant differences were not found between Chinese PMF patients with mutants CALR and JAK2V617F in terms of laboratory features. Interestingly, patients with CALR mutations showed markedly decreased levels of leukocyte alkaline phosphatase (LAP) expression, whereas those with JAK2V617F mutation presented with elevated levels. Overall, a lower mutant rate of CALR gene and a higher triple-negative rate were identified in the cohort of Chinese patients with MPNs. This result indicates that an undiscovered mutant gene may have a significant role in these patients. Moreover, these pathological features further imply that the disease biology varies considerably between mutants CALR and JAK2V617F.