Genetic Ablation of the ClC-2 Cl- Channel Disrupts Mouse Gastric Parietal Cell Acid Secretion

The present studies were designed to examine the effects of ClC-2 ablation on cellular morphology, parietal cell abundance, H/K ATPase expression, parietal cell ultrastructure and acid secretion using WT and ClC-2-/- mouse stomachs. Cellular histology, morphology and proteins were examined using ima...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-09, Vol.10 (9), p.e0138174-e0138174
Main Authors: Nighot, Meghali P, Nighot, Prashant K, Ma, Thomas Y, Malinowska, Danuta H, Shull, Gary E, Cuppoletti, John, Blikslager, Anthony T
Format: Article
Language:English
Subjects:
Ablation
Acids
Animals
ATPases
Biological Transport
Carbachol
Cell Count
Cell number
Chloride Channels - genetics
Chlorides
Cytology
Cytoplasmic Vesicles - metabolism
Digestion - genetics
Digestion - physiology
Electron microscopy
Fluorescent Antibody Technique
Gastric Acid - metabolism
Gastric glands
Gastric juice
Genetic research
Glands
H(+)-K(+)-Exchanging ATPase - biosynthesis
H(+)-K(+)-Exchanging ATPase - metabolism
Histamine
Histology
Imaging techniques
Immunofluorescence
Kinases
Measurement methods
Mice
Mice, Knockout
Microscopy, Electron
Mucosa
Mucous membrane
Parietal cells
Parietal Cells, Gastric - metabolism
Parietal Cells, Gastric - ultrastructure
Pepsinogen A - metabolism
Proteins
Rodents
Ultrastructure
Veterinary colleges
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The present studies were designed to examine the effects of ClC-2 ablation on cellular morphology, parietal cell abundance, H/K ATPase expression, parietal cell ultrastructure and acid secretion using WT and ClC-2-/- mouse stomachs. Cellular histology, morphology and proteins were examined using imaging techniques, electron microscopy and western blot. The effect of histamine on the pH of gastric contents was measured. Acid secretion was also measured using methods and secretagogues previously established to give maximal acid secretion and morphological change. Compared to WT, ClC-2-/- gastric mucosal histological organization appeared disrupted, including dilation of gastric glands, shortening of the gastric gland region and disorganization of all cell layers. Parietal cell numbers and H/K ATPase expression were significantly reduced by 34% (P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0138174