Increased Urinary Exosomal MicroRNAs in Patients with Systemic Lupus Erythematosus

There is increased interest in using microRNAs (miRNAs) as biomarkers in different diseases. Present in body fluids, it is controversial whether or not they are mainly enclosed in exosomes, thus we studied if urinary miRNAs are concentrated inside exosomes and if the presence of systemic lupus eryth...

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Bibliographic Details
Published in:PloS one 2015-09, Vol.10 (9), p.e0138618-e0138618
Main Authors: Perez-Hernandez, Javier, Forner, Maria J, Pinto, Carolina, Chaves, Felipe J, Cortes, Raquel, Redon, Josep
Format: Article
Language:English
Subjects:
Adult
Autoimmune diseases
Biological markers
Biomarkers
Biomarkers - urine
Biomedical research
Blood
Body fluids
Cancer
Case-Control Studies
Chronic conditions
Diabetes
Exosomes
Exosomes - metabolism
Female
Gene expression
Genetic aspects
Humans
Kidney diseases
Lupus
Lupus Erythematosus, Systemic - urine
Lupus nephritis
Lupus Nephritis - metabolism
Lupus Nephritis - urine
Male
MicroRNA
MicroRNAs
MicroRNAs - urine
Middle Aged
miRNA
Nephritis
Nutrition
Obesity
Patients
Systemic lupus erythematosus
Transfer RNA
Ultracentrifugation
Urine
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Summary:There is increased interest in using microRNAs (miRNAs) as biomarkers in different diseases. Present in body fluids, it is controversial whether or not they are mainly enclosed in exosomes, thus we studied if urinary miRNAs are concentrated inside exosomes and if the presence of systemic lupus erythematosus with or without lupus nephritis modifies their distribution pattern. We quantified specific miRNAs in urine of patients with systemic lupus erythematosus (n = 38) and healthy controls (n = 12) by quantitative reverse-transcription PCR in cell-free urine, exosome-depleted supernatant and exosome pellet obtained by ultracentrifugation. In control group, miR-335* and miR-302d were consistently higher in exosomes than in exosome-depleted supernatant, and miR-200c and miR-146a were higher in cell-free fraction. In lupus patients, all urinary miRNAs tested were mainly in exosomes with lower levels outside them (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0138618